Recombinant Drosophila ananassae Protein kintoun (Ppi20), partial

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In Stock
Cat. No.
BT54968
Source
Yeast
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54968
Source
E.coli
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54968
Source
E.coli
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54968
Source
Baculovirus
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Purity
>85% (SDS-PAGE)
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Cat. No.
BT54968
Source
Mammalian cell
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Purity
>85% (SDS-PAGE)
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Description

Overview of Protein Kintoun (Ppi20)

Kintoun (Ppi20), also known as dynein assembly factor 2, is a conserved protein involved in cytoplasmic dynein assembly and axonemal function. In Drosophila, it plays roles in spermatogenesis, neuronal development, and ciliary motility. Recombinant versions are used to study these processes in vitro .

Key Features of Recombinant Drosophila Kintoun Proteins

While D. ananassae-specific data is unavailable, product specifications for D. sechellia kintoun (UniProt: B4HR78) provide a template:

ParameterDetails
Product CodeCSB-EP003132DMG-B (E. coli-derived), CSB-YP003132DMG (yeast-derived)
Expression SystemE. coli (EP) or yeast (YP)
Purity>85% (SDS-PAGE verified)
Sequence CoveragePartial (residues 1–838)
TagDetermined during manufacturing (e.g., His-tag)
Storage-20°C short-term; -80°C for long-term with 50% glycerol
ReconstitutionSterile water; avoid repeated freeze-thaw cycles

Functional and Research Applications

  • Dynein Complex Assembly: Kintoun facilitates dynein motor complex formation, essential for intracellular transport .

  • Axonemal Function: Critical for sperm flagella and sensory cilia motility in Drosophila models.

  • PP1 Phosphatase Interaction: Modulates phosphatase activity in cell cycle regulation .

Limitations and Research Gaps

No peer-reviewed studies on D. ananassae Ppi20 were identified. Current knowledge is extrapolated from:

  • Orthologs: Functional studies in D. melanogaster and D. sechellia .

  • Technical Constraints: Recombinant kintoun proteins require eukaryotic systems for proper folding, limiting bacterial expression efficacy .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. For dry ice shipping, please contact us in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us and we will prioritize its development.
Synonyms
Nop17l; Ppi20; GF11794Protein kintoun; Dynein assembly factor 2; axonemal homolog; PP1-interacting protein 20
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Drosophila ananassae (Fruit fly)
Target Names
Nop17l
Uniprot No.
B3MG50

Target Background

Function
Required for cytoplasmic pre-assembly of axonemal dyneins, playing a central role in cilia and flagella motility. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport to the ciliary compartment.
Database Links

KEGG: dan:Dana_GF11794

STRING: 7217.FBpp0114986

Protein Families
PIH1 family, Kintoun subfamily
Subcellular Location
Cytoplasm.

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