Recombinant Drosophila grimshawi Flap endonuclease 1 (Fen1)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please contact us in advance as additional charges apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
Fen1; GH21157Flap endonuclease 1; FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-388
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Target Names
Target Protein Sequence
MGILGLSKLI ADLAPLAIRE SEIKNFFGRK VAIDASMCLY QFLIAVRSEG AQLAAVNGDP
TSHLMGMFYR TIRLLDNGIK PVYVFDGAPP DMKSGELAKR AERRDEAEKA LKAATEAGDE
AQIEKFNRRL VRVTKEHSRE AKELLKLMGV PYVDAPCEAE AQCAALVKAG KVYATATEDM
DALTFGSCKL LRYLTYSEAR KMPVKEFSYD KVLQGLELTS KEFIDLCILM GCDYCESIRG
VGPKRAIELI KSYRDIETIL ENIDTNKYAV PENWNYKRAR ELFIEPDVTD ASTIDLKWTD
PDEDGLVQFL CGDRQFNEER VRNGAKKLLK SKQAQTQVRL DSFFKTLPSS PNAIAAAKRK
AEESKKSANS KKAKIGGGSG AGRGRRPK
Uniprot No.
Target Background
Function
Flap endonuclease 1 (Fen1) is a structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During replication, it cleaves the 5'-overhanging flap created by displacement synthesis when DNA polymerase encounters a downstream Okazaki fragment. It enters the flap from the 5'-end, tracks, and cleaves at the base, leaving a nick for ligation. Fen1 is also involved in long patch base excision repair (LP-BER) by cleaving within the AP site-terminated flap. It acts as a genome stabilizer, preventing flaps from forming structures that lead to duplications and deletions. It also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA and exhibits RNase H activity. Additionally, it's involved in rDNA replication and repair and mitochondrial DNA repair.
Database Links
KEGG: dgr:Dgri_GH21157
STRING: 7222.FBpp0155063
Protein Families
XPG/RAD2 endonuclease family, FEN1 subfamily
Subcellular Location
Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion.
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