Recombinant Drosophila melanogaster Putative gustatory receptor 59d (Gr59d)
Description
Molecular Identity and Production
Gr59d (Gene ID: CG30330) is a recombinant protein belonging to the insect gustatory receptor (GR) family. It is produced in heterologous systems such as E. coli, yeast, baculovirus, or mammalian cells, achieving ≥85% purity via SDS-PAGE . The receptor shares gene aliases (GR59D.1, 59D1) and structural features with other GRs, including seven transmembrane domains and a ligand-gated ion channel architecture .
Research Applications
Gr59d is utilized in two primary contexts:
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Antibody Development: A rabbit polyclonal antibody (IgG isotype) targets Gr59d for immunohistochemistry and Western blotting, validated in Drosophila tissues .
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Ligand Screening: Recombinant Gr59d can be expressed in heterologous systems (e.g., HEK293 cells) to identify activating or inhibitory compounds, paralleling methods used for Gr59c .
Unresolved Questions and Future Directions
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Ligand Specificity: No direct ligands have been identified for Gr59d, unlike related receptors (e.g., Gr5a for sugars, Gr59c for lobeline) .
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Cellular Context: GRs often require co-receptors for functionality . Identifying Gr59d’s interaction partners is critical.
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Behavioral Role: In vivo studies using Gr59d mutants or knockouts could clarify its impact on feeding or avoidance behaviors.
Comparative Analysis with Related Receptors
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it during order placement. We will accommodate your request to the best of our ability.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance. Additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
