Recombinant Enterobacter sp. UPF0060 membrane protein Ent638_1931 (Ent638_1931)
Description
Production Methods
Ent638_1931 is primarily produced in E. coli due to its cost-effectiveness and high yield . Alternative expression systems include:
| Host System | Advantages |
|---|---|
| Yeast | Faster turnaround, moderate post-translational modifications |
| Insect Cells | Advanced folding and eukaryotic modifications (e.g., glycosylation) |
| Mammalian Cells | Full post-translational processing for functional studies |
For research requiring native-like folding or activity validation, insect or mammalian systems are recommended .
Applications in Research
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Structural Studies: The protein’s small size (12.8 kDa) and solubility make it suitable for X-ray crystallography or NMR .
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Antigen Development: Available in ELISA-ready formats for antibody production and immunoassays .
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Membrane Protein Interaction Studies: Potential use in probing bacterial membrane dynamics, though its specific biological role remains uncharacterized .
Research Considerations
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Functional Data Gap: No confirmed enzymatic or receptor activities are documented for Ent638_1931 .
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Pathway Associations: Preliminary data suggest involvement in uncharacterized metabolic or regulatory pathways .
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Interactome: Direct protein-protein interactions remain unexplored but could be studied via pull-down assays .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during ordering for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
If you require a specific tag, please inform us; we will prioritize its development.
Target Background
KEGG: ent:Ent638_1931
STRING: 399742.Ent638_1931
