Recombinant Enterococcus faecalis Asparagine--tRNA ligase (asnS)
Description
Introduction to Recombinant Enterococcus faecalis Asparagine--tRNA Ligase (asnS)
Recombinant Enterococcus faecalis Asparagine--tRNA ligase, also known as asnS, is an enzyme that plays a crucial role in the process of protein synthesis by catalyzing the attachment of asparagine to its corresponding transfer RNA (tRNA). This enzyme belongs to the family of aminoacyl-tRNA synthetases, which are essential for ensuring the accuracy of protein synthesis by linking amino acids to their appropriate tRNA molecules.
Function and Mechanism
Asparagine--tRNA ligase catalyzes the following chemical reaction:
This reaction involves the hydrolysis of ATP to AMP and diphosphate, with the simultaneous formation of L-asparaginyl-tRNA, which is then used in protein synthesis to incorporate asparagine into polypeptide chains.
Characteristics of Recombinant asnS
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Source: The recombinant form of this enzyme is typically produced in bacterial systems like E. coli for ease of expression and purification.
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Purity and Stability: The purity of recombinant asnS is often greater than 85%, as determined by SDS-PAGE, and its stability can vary depending on storage conditions, such as temperature and buffer composition .
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Sequence and Structure: The enzyme sequence includes specific motifs that are conserved among aminoacyl-tRNA synthetases, facilitating its interaction with ATP and tRNA.
Research Findings
While specific research on Enterococcus faecalis asnS might be limited, studies on aminoacyl-tRNA synthetases in general highlight their critical roles in protein synthesis and potential applications in biotechnology and medicine. For instance, mutations or alterations in these enzymes can lead to errors in protein synthesis, affecting cellular function and potentially leading to disease states.
Table 1: Characteristics of Recombinant Asparagine--tRNA Ligase (asnS)
| Characteristic | Description |
|---|---|
| Source | Produced in E. coli |
| Purity | >85% (SDS-PAGE) |
| Stability | Dependent on storage conditions |
| Sequence | Conserved motifs for ATP and tRNA interaction |
Table 2: Comparison of Aminoacyl-tRNA Synthetases
| Enzyme | Class | Function |
|---|---|---|
| AsnRS | II | Charges tRNA with asparagine |
| ProRS | II | Charges tRNA with proline, exhibits editing activity |
| TrpRS | I | Charges tRNA with tryptophan, interacts with NOS in some bacteria |
Product Specs
Note: We will prioritize shipping the format currently in stock. However, please specify any format requirements in your order notes, and we will fulfill your request to the best of our ability.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior arrangement and incurs additional charges.
Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C. Lyophilized formulations have a 12-month shelf life at -20°C/-80°C.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: efa:EF2371
STRING: 226185.EF2371
