Recombinant Enterococcus faecalis Ethanolamine ammonia-lyase light chain (eutC)

Introduction to Ethanolamine Ammonia-Lyase

Ethanolamine ammonia-lyase is an enzyme crucial for the catabolism of ethanolamine, a compound derived from cell membranes. This enzyme is composed of two subunits: a heavy chain and a light chain. In bacteria like Enterococcus faecalis, the ethanolamine ammonia-lyase is encoded by the eutB and eutC genes, where eutC represents the light chain. The recombinant form of this enzyme, specifically the light chain from Enterococcus faecalis, is of interest for its role in ethanolamine metabolism.

Function of Ethanolamine Ammonia-Lyase

Ethanolamine ammonia-lyase catalyzes the breakdown of ethanolamine into acetaldehyde and ammonia. This reaction is crucial for bacteria that utilize ethanolamine as a source of carbon and nitrogen. The enzyme requires adenosylcobalamin (AdoCbl) as a coenzyme and operates within specialized bacterial microcompartments known as metabolosomes.

Ethanolamine Utilization in Bacteria

Ethanolamine is particularly prevalent in environments rich in cell membranes, such as the gastrointestinal tract. Bacteria capable of catabolizing ethanolamine, like Enterococcus faecalis, possess a complex set of genes known as the eut operon. This operon includes eutB and eutC, which encode the subunits of ethanolamine ammonia-lyase, as well as accessory genes that enhance ethanolamine breakdown .

Research Findings and Data

While specific data on the recombinant eutC from Enterococcus faecalis is not readily available, studies on ethanolamine ammonia-lyase in other bacteria provide valuable insights into its function and importance. For instance, in Salmonella enterica, the ethanolamine ammonia-lyase is localized within a metabolosome, where it interacts with other enzymes to facilitate ethanolamine catabolism .

Table: Ethanolamine Ammonia-Lyase Subunits and Their Functions

Table: Key Components of Ethanolamine Catabolism

References Search results did not provide specific information on the recombinant Enterococcus faecalis ethanolamine ammonia-lyase light chain (eutC). However, general information on ethanolamine ammonia-lyase and its role in bacteria was derived from related studies. Ethanolamine Utilization in Bacteria. PMC. This reference is not relevant to the topic of ethanolamine ammonia-lyase. Localization and interaction studies of the Salmonella enterica ethanolamine ammonia-lyase (EutBC), its reactivase (EutA), and the EutT corrinoid adenosyltransferase. PubMed. This reference is not relevant to the topic of ethanolamine ammonia-lyase. Localization and interaction studies of the Salmonella enterica ethanolamine ammonia-lyase (EutBC), its reactivase (EutA), and the EutT corrinoid adenosyltransferase. PMC. Rational Use of Pancreatic Enzymes for Pancreatic Insufficiency and Malabsorption. PMC. Ethanolamine Utilization in Bacterial Pathogens: Roles and Regulation. PMC.