Recombinant Enterococcus faecalis Ferrochelatase (hemH)

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In Stock
Cat. No.
BT2434080
Source
Yeast
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434080
Source
E.coli
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT2434080
Source
E.coli
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434080
Source
Baculovirus
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT2434080
Source
Mammalian cell
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Purity
>85% (SDS-PAGE)
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Description

Role of Ferrochelatase in Heme Biosynthesis

Ferrochelatase is the final enzyme in the heme biosynthetic pathway. It plays a critical role by converting protoporphyrin IX into heme by inserting ferrous iron. In bacteria like Enterococcus faecalis, which do not synthesize heme de novo, ferrochelatase can utilize exogenous protoporphyrin IX to produce heme for hemoproteins .

Recombinant Ferrochelatase in Enterococcus faecalis

While specific studies on recombinant Enterococcus faecalis ferrochelatase (hemH) are scarce, the enzyme's role in utilizing protoporphyrin IX to produce heme is crucial for the bacterium's ability to form hemoproteins. This capability allows Enterococcus faecalis to adapt to environments where heme is not readily available.

Table 1: Key Features of Ferrochelatase in Bacteria

FeatureDescription
FunctionInserts ferrous iron into protoporphyrin IX to form heme.
Role in E. faecalisUtilizes exogenous protoporphyrin IX to produce heme for hemoproteins.
Potential ApplicationsBiotechnological production of heme-containing compounds.

References

  1. Bryan-Jones, D. G., & Whittenbury, R. (1969). Hemoprotein synthesis by bacteria. Journal of General Microbiology, 58(3), 247-260.

  2. Frankenberg, L. (n.d.). Hemoproteins of Enterococcus faecalis. Lucris.

  3. Saillant, et al. (2021). A Novel Enterococcus faecalis Heme Transport Regulator (FhtR). PMC.

Product Specs

Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery timelines.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is specifically requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, and can serve as a guideline for customers.
Shelf Life
Shelf life depends on several factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The specific tag type is determined during production. If a particular tag is required, please inform us, and we will prioritize its development.
Synonyms
hemH; EF_1989Ferrochelatase; EC 4.99.1.1; Heme synthase; Protoheme ferro-lyase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-313
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Enterococcus faecalis (strain ATCC 700802 / V583)
Target Names
hemH
Target Protein Sequence
MKRTGILLVN LGTPKDSSKT EVRKYLKTFL SDRRVIKIHP IIWKPILNGI ILNIRPKKSA KLYQKICTEN GFPLLEYTEK QMENLKNICP EVEVTIGMSY SEPSIETALD TLLSKEIEEL NVIPMYPQYS GTTVGSVFDS VMNYFIKSDR IVDIKFIRSF YNNPQYIDYF SKKINEALNE SPIDAIVFSY HGIPMSYVKD GDNYPKECTK TTKLIMDKLG DIRYYQTYQS KFGPSEWLKP ATDDTLKKLP SKGIKNILIV APGFVVDCLE TIEELEHENR NYFLENGGEV YKYVHPFNGD IEFAKLVKDI ISL
Uniprot No.
Q833G5

Target Background

Function

Involved in coproporphyrin-dependent heme b biosynthesis. Catalyzes the insertion of ferrous iron into coproporphyrin III, forming Fe-coproporphyrin III.

Database Links

KEGG: efa:EF1989

STRING: 226185.EF1989

Protein Families
Ferrochelatase family
Subcellular Location
Cytoplasm.

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