Recombinant Enterococcus phage phiEF24C Virion protein 3

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Cat. No.
BT37738
Source
Yeast
Synonyms
40Virion protein 3; Gene product 40; gp40
Purity
>85% (SDS-PAGE)
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Cat. No.
BT37738
Source
E.coli
Synonyms
40Virion protein 3; Gene product 40; gp40
Purity
>85% (SDS-PAGE)
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Cat. No.
BT37738
Source
E.coli
Synonyms
40Virion protein 3; Gene product 40; gp40
Purity
>85% (SDS-PAGE)
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Cat. No.
BT37738
Source
Baculovirus
Synonyms
40Virion protein 3; Gene product 40; gp40
Purity
>85% (SDS-PAGE)
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Cat. No.
BT37738
Source
Mammalian cell
Synonyms
40Virion protein 3; Gene product 40; gp40
Purity
>85% (SDS-PAGE)
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Description

Origin and Biological Context

PhiEF24C is a virulent Myoviridae phage isolated for its therapeutic potential against E. faecalis infections . Its linear double-stranded DNA genome (142,072 bp) encodes 221 open reading frames (ORFs), including structural proteins essential for virion assembly and host recognition . Virion Protein 3 corresponds to a key structural component implicated in host adsorption, as identified through genomic and proteomic analyses .

Adsorption Efficiency

A spontaneous mutant phage, phiEF24C-P2, harboring a point mutation in orf31 (encoding Virion Protein 3), demonstrated enhanced adsorption rates compared to the wild-type phage :

Phage VariantAdsorption Rate (% adsorbed in 10 min)Host Strain
Wild-type phiEF24C58%E. faecalis EF14
Mutant phiEF24C-P292%E. faecalis EF14
Wild-type phiEF24C12%E. faecalis VRE2
Mutant phiEF24C-P267%E. faecalis VRE2

This mutation (Glu798Lys in ORF31) improved binding to bacterial surfaces, particularly in low-sensitivity strains like VRE2 .

Therapeutic Relevance

  • In Vivo Efficacy: PhiEF24C reduced mortality in sepsis mouse models infected with E. faecalis, even at low multiplicities of infection (MOI = 0.01) .

  • Safety Profile: Genomic analysis confirmed the absence of virulence factors, antibiotic resistance genes, or lysogeny-associated integrases .

  • Diagnostic Tools: The C-terminal domain of phiEF24C endolysin has been engineered into magnetic bead complexes for specific detection of E. faecalis in mixed cultures .

  • Phage Engineering: Recombinant Virion Protein 3 serves as a target for optimizing phage adsorption kinetics, as demonstrated in phiEF24C-P2 .

Challenges and Limitations

  • Host Sensitivity Variability: Wild-type phiEF24C requires higher concentrations to rescue mice infected with low-sensitivity VRE strains .

  • Structural Complexity: The large size (~203 kDa) and conformational flexibility of Virion Protein 3 complicate recombinant production .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult your local distributor for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
40Virion protein 3; Gene product 40; gp40
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-181
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Enterococcus phage phiEF24C (Enterococcus bacteriophage phi-EF24C)
Target Names
40
Target Protein Sequence
AIATNNSRV YASLQLKNKQ DSMYLAIGKT TPWTNEDAPP APDPTTTTLT EVIGYKKVAR VSLCREYLPS DDSKYPVVSY GSRKFTLIPD EDGYKEQAWM VYVEAEITGD ELPTGTFRQV GIHTDLVSKA SSEKKALLPT DVTDAGILQF FENRQQQNRT SDVILKEKFI ITMENKKSVK Q
Uniprot No.
P85227

Target Background

Function
Putative baseplate protein.
Database Links

KEGG: vg:5666497

Protein Families
Tevenvirinae baseplate structural protein gp8 family
Subcellular Location
Virion.

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