Recombinant Equus zebra Hexokinase-2 (HK2), partial
Shipped with Ice Packs 
In Stock 
Description
Key Features of Recombinant HK2:
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Catalytic Activity: Requires ATP and glucose, with product inhibition by glucose-6-phosphate .
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Isoform Specificity: Unlike HK1, both N-terminal and C-terminal halves of HK2 are catalytically active, though the N-terminal half exhibits higher activity .
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Mitochondrial Binding: Critical for full glycolytic function; truncation of mitochondrial binding domains reduces activity .
Recombinant HK2 Production
Recombinant HK2 is typically expressed in Escherichia coli (E. coli) or wheat germ systems. Key parameters from available datasets include:
Research Applications
Recombinant HK2 is widely used to study metabolic reprogramming in cancer and therapeutic targeting:
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Cancer Biology:
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Signaling Pathways:
Therapeutic Implications
Product Specs
Form
Lyophilized powder. We will preferentially ship the available format. For specific format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs. For dry ice shipment, contact us in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag preference, please inform us.
Synonyms
HK2; Hexokinase-2; EC 2.7.1.1; Hexokinase type II; HK II
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Equus zebra (Mountain zebra)
Target Names
Uniprot No.
Target Background
Function
Catalyzes hexose (e.g., D-glucose, D-fructose) phosphorylation to hexose 6-phosphate. Initiates glycolysis by phosphorylating D-glucose to D-glucose 6-phosphate. Maintains outer mitochondrial membrane integrity by preventing apoptogenic molecule release and subsequent apoptosis.
Protein Families
Hexokinase family
Subcellular Location
Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol.
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