Recombinant Erwinia amylovora UDP-galactose-lipid carrier transferase (amsG)
Description
Introduction to AmsG
AmsG (encoded by the amsG gene) is a UDP-galactose-lipid carrier transferase that catalyzes the transfer of galactose from UDP-galactose to a lipid carrier during amylovoran biosynthesis . Amylovoran serves as a virulence factor, enabling bacterial colonization and obstruction of plant vascular systems . Recombinant AmsG refers to the enzyme produced via heterologous expression systems for functional and structural studies.
Functional Role in Amylovoran Biosynthesis
AmsG operates within the 12-gene ams cluster (amsA–amsL) . Key functions include:
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Subunit Assembly: Anchors galactose to a lipid carrier, forming repeating oligosaccharide units of amylovoran .
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Regulation: Expression is modulated by cyclic di-GMP (c-di-GMP) via CsrD, which stabilizes amsG mRNA .
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Virulence Link: Mutants lacking amsG show reduced amylovoran production and impaired pathogenicity .
Recombinant Production
Recombinant AmsG is typically expressed in Escherichia coli for biochemical studies:
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Cloning: The amsG gene is amplified from E. amylovora and ligated into expression vectors (e.g., pET series) .
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Purification: Affinity chromatography tags (e.g., His-tag) enable high-yield isolation .
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Activity Assays: Measured via UDP-galactose consumption or lipid-linked galactose detection .
Table 1: Key Studies on AmsG
Table 2: Impact of AmsG on Virulence
| Condition | Effect on Amylovoran | Pathogenicity |
|---|---|---|
| amsG overexpression | Increased EPS production | Enhanced tissue necrosis . |
| amsG knockout | Reduced EPS synthesis | Attenuated disease progression . |
Significance and Applications
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Biocontrol Strategies: Targeting AmsG could disrupt amylovoran synthesis, reducing bacterial virulence .
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Agricultural Research: CRISPR-based genotyping of amsG aids in tracking E. amylovora outbreaks .
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Enzyme Engineering: Recombinant AmsG serves as a model for studying glycosyltransferase mechanisms .
Challenges and Future Directions
Product Specs
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Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
