Recombinant Escherichia coli 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (ubiF)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. Note: We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times may vary based on purchasing method and location. Please consult your local distributor for specific delivery times. Note: All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees apply.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C. The lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
ubiF; yleB; b0662; JW0659; 3-demethoxyubiquinol 3-hydroxylase; EC 1.14.99.60; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-391
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia coli (strain K12)
Target Names
ubiF
Target Protein Sequence
MTNQPTEIAI VGGGMVGGAL ALGLAQHGFA VTVIEHAEPA PFVADSQPDV RISAISAASV
SLLKGLGVWD AVQAMRCHPY RRLETWEWET AHVVFDAAEL KLPLLGYMVE NTVLQQALWQ
ALEAHPKVTL RVPGSLIALH RHDDLQELEL KGGEVIRAKL VIGADGANSQ VRQMAGIGVH
AWQYAQSCML ISVQCENDPG DSTWQQFTPD GPRAFLPLFD NWASLVWYDS PARIRQLQNM
NMAQLQAEIA KHFPSRLGYV TPLAAGAFPL TRRHALQYVQ PGLALVGDAA HTIHPLAGQG
VNLGYRDVDA LIDVLVNARS YGEAWASYPV LKRYQMRRMA DNFIMQSGMD LFYAGFSNNL
PPLRFMRNLG LMAAERAGVL KRQALKYALG L
Uniprot No.
Target Background
Function
Catalyzes the hydroxylation of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol during ubiquinone biosynthesis.
Gene References Into Functions
1. Studies have shown that sucB and ubiF mutants, deficient in energy production, exhibit reduced persister cell survival and increased susceptibility to antibiotics and stress (PMID: 20041955).
2. This protein functions as a 2-octoprenyl-3-methyl-6-methoxy-1,4-benzoquinol oxygenase (PMID: 10802164).
Database Links
KEGG: ecj:JW0659
STRING: 316385.ECDH10B_0731
Protein Families
UbiH/COQ6 family
Subcellular Location
Cytoplasm.
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