Recombinant Escherichia coli O127:H6 Peptide chain release factor 1 (prfA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies by purchase method and location. Consult your local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Request dry ice shipment in advance for an extra fee.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form shelf life is generally 6 months at -20°C/-80°C. Lyophilized form shelf life is generally 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
prfA; E2348C_1334; Peptide chain release factor 1; RF-1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-360
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia coli O127:H6 (strain E2348/69 / EPEC)
Target Names
prfA
Target Protein Sequence
MKPSIVAKLE ALHERHEEVQ ALLGDAQTIA DQERFRALSR EYAQLSDVSR CFTDWQQVQE
DIETAQMMLD DPEMREMAQD ELREAKEKSE QLEQQLHVLL LPKDPDDERN AFLEVRAGTG
GDEAALFAGD LFRMYSRYAE ARRWRVEIMS ASEGEHGGYK EIIAKISGDG VYGRLKFESG
GHRVQRVPAT ESQGRIHTSA CTVAVMPELP DAELPDINPA DLRIDTFRSS GAGGQHVNTT
DSAIRITHLP TGIVVECQDE RSQHKNKAKA LSVLGARIHA AEMAKRQQAE ASTRRNLLGS
GDRSDRNRTY NFPQGRVTDH RINLTLYRLD EVMEGKLDML IEPIIQEHQA DQLAALSEQE
Uniprot No.
Target Background
Function
Peptide chain release factor 1 terminates translation in response to the stop codons UAG and UAA.
Database Links
KEGG: ecg:E2348C_1334
Protein Families
Prokaryotic/mitochondrial release factor family
Subcellular Location
Cytoplasm.
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