Recombinant Escherichia coli O139:H28 GTPase Era (era)

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Cat. No.

BT33790

Source

Yeast

Synonyms

era; EcE24377A_2852GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33790

Source

E.coli

Synonyms

era; EcE24377A_2852GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33790

Source

E.coli

Synonyms

era; EcE24377A_2852GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33790

Source

Baculovirus

Synonyms

era; EcE24377A_2852GTPase Era

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33790

Source

Mammalian cell

Synonyms

era; EcE24377A_2852GTPase Era

Purity

>85% (SDS-PAGE)

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Description

Recombinant Production and Biochemical Properties

Recombinant Era is typically expressed in E. coli with affinity tags (e.g., His-tag) for purification. Key characteristics include:

Property	Details
Expression Host	E. coli BL21(DE3) or similar strains
Purification Method	Affinity chromatography (e.g., Ni-NTA for His-tagged proteins)
Molecular Weight	~32 kDa (monomer)
GTPase Activity	RNA-dependent; stimulated by K+ ions (up to 10-fold activation)
RNA Binding	Associates with 16S rRNA precursors; critical for 3′-end maturation

Note: RNA-bound Era exhibits higher GTPase activity, while RNA-free forms require exogenous RNA or metabolites (e.g., acetate) for activation .

Role in Ribosome Biogenesis

Era stabilizes immature 16S rRNA, enabling proper processing by exonucleases (RNase II, RNase PH) and endonuclease YbeY .
Overexpression of Era partially suppresses 16S rRNA defects in ΔybeY strains, improving ribosome assembly .

Suppressor Mutations

T99I Mutation: Restores 16S rRNA processing in ΔybeY strains by enhancing Era’s interaction with rRNA precursors, though it fails to rescue temperature sensitivity .
E200K Mutation: Disrupts KH domain function, exacerbating ribosome biogenesis defects .

Interactions with Chaperones and Ribosomal Proteins

GroEL/ES: Binds Era(T99I) mutants, potentially stabilizing misfolded intermediates during ribosome assembly .
RbfA and YbeY: Genetic interactions suggest Era collaborates with these factors to coordinate platform maturation in the 30S subunit .

Implications for Antibiotic Development

Era’s essentiality and conserved role in ribosome assembly make it a potential antimicrobial target. Inhibiting its GTPase activity or rRNA binding could disrupt bacterial proliferation .

Unresolved Questions and Research Gaps

GTPase Activation: The identity of Era’s GTPase-activating protein (GAP) remains unknown .
Strain-Specific Variations: Limited data exist on Era from E. coli O139:H28; most studies use K-12 or other lab strains .

Product Specs

Form

Lyophilized powder. We will preferentially ship the available format. For specific format requirements, please note them when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us for preferential development.

Synonyms

era; EcE24377A_2852GTPase Era

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-301

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Escherichia coli O139:H28 (strain E24377A / ETEC)

Target Names

era

Target Protein Sequence

MSIDKSYCGF IAIVGRPNVG KSTLLNKLLG QKISITSRKA QTTRHRIVGI HTEGAYQAIY VDTPGLHMEE KRAINRLMNK AASSSIGDVE LVIFVVEGTR WTPDDEMVLN KLRDGKAPVI LAVNKVDNVQ EKADLLPHLQ FLASQMNFLD IVPISAETGL NVDTIAAIVR KHLPEATHHF PEDYITDRSQ RFMASEIIRE KLMRFLGAEL PYSVTVEIER FVSNERGGYD INGLILVERE GQKKMVIGNK GAKIKTIGIE ARKDMQEMFE APVHLELWVK VKSGWADDER ALRSLGYVDD L

Uniprot No.

A7ZQ10

Target Background

Function

An essential GTPase that binds GDP and GTP, with rapid nucleotide exchange. Involved in 16S rRNA processing, 30S ribosomal subunit biogenesis, and possibly cell cycle regulation and energy metabolism.

Database Links

KEGG: ecw:EcE24377A_2852

Protein Families

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Era GTPase family

Subcellular Location

Cytoplasm. Cell inner membrane; Peripheral membrane protein.

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Recombinant Escherichia coli O139:H28 GTPase Era (era)

Description

Recombinant Production and Biochemical Properties

Role in Ribosome Biogenesis

Suppressor Mutations

Interactions with Chaperones and Ribosomal Proteins

Implications for Antibiotic Development

Unresolved Questions and Research Gaps

Product Specs

Target Background

Quick Inquiry

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Service