Recombinant Escherichia coli O17:K52:H18 Glucose-6-phosphate isomerase (pgi)

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Cat. No.
BT33228
Source
Yeast
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33228
Source
E.coli
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33228
Source
E.coli
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33228
Source
Baculovirus
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Purity
>85% (SDS-PAGE)
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Cat. No.
BT33228
Source
Mammalian cell
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Purity
>85% (SDS-PAGE)
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Description

Introduction and Overview

Glucose-6-phosphate isomerase (pgi) is a key enzyme in glycolysis, catalyzing the reversible isomerization of glucose-6-phosphate (G6P) to fructose-6-phosphate (F6P) . In Escherichia coli, this enzyme is critical for maintaining metabolic flux balance between the glycolytic pathway and the pentose phosphate pathway (PPP) . The recombinant form of E. coli pgi, particularly from the O17:K52:H18 strain, is studied for its biochemical properties, structural insights, and applications in metabolic engineering.

Biochemical Role and Significance

Function in Central Metabolism

  • Pgi facilitates the interconversion of G6P and F6P, enabling carbon flux through glycolysis and PPP .

  • Loss of pgi reroutes metabolism to the PPP, increasing NADPH production and activating compensatory pathways like the glyoxylate shunt .

Impact of Deletion

  • Δpgi mutants exhibit slow growth, reliance on PPP, and acetate/formate overflow metabolism .

  • Adaptive evolution in Δpgi strains involves mutations in rpoS, udhA, and pntAB to restore redox balance .

Recombinant Production and Characterization

Recombinant pgi is produced via heterologous expression in systems like E. coli or yeast. Key product specifications include:

ParameterValueSource
Host OrganismE. coli or yeast
TagHis-tag, Avi-tag (biotinylated)
Purity≥85% (SDS-PAGE)
Protein Length309–553 amino acids (varies by construct)
Storage BufferTris-based buffer with 50% glycerol

Functional Validation

  • Recombinant pgi retains catalytic activity similar to native E. coli pgi .

  • Heterologous PGIs (e.g., from archaea) can substitute for E. coli pgi, albeit with reduced growth rates .

Applications in Research and Biotechnology

Metabolic Engineering

  • Pgi modulation is explored to optimize biofuel production (e.g., ethanol) and reduce acetate overflow .

  • Deletion of pgi enhances PPP-dependent pathways, useful for synthesizing NADPH-rich metabolites .

Drug Target Potential

  • Pgi inhibitors disrupt sugar metabolism in pathogens like Aspergillus flavus, affecting virulence .

  • Structural studies of pgi guide rational drug design for targeting metabolic vulnerabilities .

Comparative Analysis with Other PGIs

FeatureE. coli pgiArchaeal pgi (e.g., Pyrococcus furiosus)
Thermal StabilityModerateHigh
Catalytic EfficiencyOptimized for glucoseAdapted to non-phosphorylated substrates
Host ExpressionE. coli (common)E. coli or heterologous systems

Functional Redundancy

  • E. coli pgi can be replaced by PGIs from diverse sources, including eukaryotes and archaea, though with varying efficiency .

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format, but if you have special requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchasing method and location. Please consult your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. For dry ice shipment, please contact us in advance; additional charges will apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C is recommended. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, while the lyophilized form is stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
pgi; ECUMN_4558Glucose-6-phosphate isomerase; GPI; EC 5.3.1.9; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-549
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Target Names
pgi
Target Protein Sequence
MKNINPTQTA AWQALQKHFD EMKDVTIADL FAKDGDRFSK FSATFDDQML VDYSKNRITE ETLAKLQDLA KECDLAGAIK SMFSGEKINR TENRAVLHVA LRNRSNTPIL VDGKDVMPEV NAVLEKMKTF SEAIISGEWK GYTGKAITDV VNIGIGGSDL GPYMVTEALR PYKNHLNMHF VSNVDGTHIA EVLKKVNSET TLFLVASKTF TTQETMTNAH SARDWFLKAA GDEKHVAKHF AALSTNAKAV GEFGIDTANM FEFWDWVGGR YSLWSAIGLS IVLSIGFDNF VELLSGAHAM DKHFSTTPAE KNLPVLLALI GIWYNNFFGA ETEAILPYDQ YMHRFAAYFQ QGNMESNGKY VDRNGNVVDY QTGPIIWGEP GTNGQHAFYQ LIHQGTKIVP CDFIAPAITH NPLSDHHQKL LSNFFAQTEA LAFGKSREVV EQEYRDQGKD PATLDYVVPF KVFEGNRPTN SILLREITPF SLGALIALYE HKIFTQGVIL NIFTFDQWGV ELGKQLANRI LPELKDDKEI SSHDSSTNGL INRYKAWRG
Uniprot No.
B7NFW8

Target Background

Function
Catalyzes the reversible isomerization between glucose-6-phosphate and fructose-6-phosphate.
Database Links

KEGG: eum:ECUMN_4558

Protein Families
GPI family
Subcellular Location
Cytoplasm.

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