Recombinant Escherichia coli O17:K52:H18 Methionyl-tRNA formyltransferase (fmt)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time may vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. Contact us in advance for dry ice shipping (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
fmt; ECUMN_3761Methionyl-tRNA formyltransferase; EC 2.1.2.9
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-315
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC)
Target Names
fmt
Target Protein Sequence
MSESLRIIFA GTPDFAARHL DALLSSGHNV VGVFTQPDRP AGRGKKLMPS PVKVLAEEKA
LPVFQPVSLR PQENQQLVAD LQADVMVVVA YGLILPKAVL EMPRLGCINV HGSLLPRWRG
AAPIQRSLWA GDAETGVTIM QMDVGLDTGD MLYKLSCPIT AEDTSGTLYD KLAELGPQGL
ITTLKQLADG TAKPEVQDET LVTYAEKLSK EEARIDWSLS AAQLERCIRA FNPWPMSWLE
IEGQPVKVWK ASVIDTATNA APGTILEANK QGIQVATGDG ILNLLSLQPA GKKAMSAQDL
LNSRREWFVP GNRLV
Uniprot No.
Target Background
Function
Attaches a formyl group to methionyl-tRNA(fMet). This formyl group plays a dual role: it promotes recognition by IF2 and prevents its use by the elongation apparatus during protein synthesis.
Database Links
KEGG: eum:ECUMN_3761
Protein Families
Fmt family
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