Recombinant Escherichia coli O8 Acyl carrier protein (acpP)

Shipped with Ice Packs

In Stock

Cat. No.

BT38547

Source

Yeast

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT38547

Source

E.coli

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT38547

Source

E.coli

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT38547

Source

Baculovirus

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT38547

Source

Mammalian cell

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

Quick Inquiry

Description

Core Function of ACP in E. coli

ACP is a central component of fatty acid synthesis (FAS) in E. coli, serving as a scaffold for acyl chain elongation. It shuttles intermediates between enzymes (e.g., acetyl-CoA carboxylase, β-ketoacyl-ACP synthases) and facilitates interactions with regulatory proteins like SpoT, which modulate (p)ppGpp synthesis under stress .

Key Features of E. coli ACP

Property	Description	Source
Phosphopantetheinylation	Post-translational modification critical for acyl chain binding
Interaction Network	Binds to SpoT, RelA, DHPS, and LpxD (lipid A biosynthesis)
Structural Flexibility	Engineering into multimeric constructs enhances functional versatility

O8 Serotype and O-Antigen Biosynthesis

The O8 serotype is defined by its mannose homopolymer O-antigen, synthesized via the ABC transporter pathway. This process involves conserved and serotype-specific mannosyltransferases (WbdC, WbdB, WbdA) .

While no studies explicitly target O8 acpP, recombinant ACPs from other species (e.g., Ralstonia, Pseudomonas) have been expressed in E. coli to study fatty acid or polyhydroxyalkanoate (PHA) synthesis .

Functional Complementation of E. coli acpP Mutants

ACP Source	Host Strain	Complementation Efficiency	Key Observations
Ralstonia	CY1877 (acpP−)	Partial (AcpP1 only)	AcpP1 supports growth; others fail
Pseudomonas	K1060 (LacIq)	Successful PHB production	Requires strong promoters

Source:

Potential Links Between O8 and ACP

The O8 O-antigen and ACP pathways are distinct, but lipid A (synthesized via LpxD, which interacts with ACP ) and lipid-linked intermediates (e.g., undecaprenyl pyrophosphate) may intersect. For example:

Lipid A Synthesis: ACP interacts with LpxD, linking fatty acid synthesis to lipopolysaccharide (LPS) assembly .
Metabolic Burden: Recombinant protein expression (e.g., AAR, PHA synthases) impacts fatty acid pathways, as shown in E. coli M15 and DH5α strains .

Research Gaps and Future Directions

O8-Specific ACP Engineering: No studies have targeted acpP from O8 strains. Potential applications include enhancing fatty acid derivatives for O-antigen or lipid A modifications.
Synthetic Biology: Tandem ACP constructs (e.g., di- or tri-domain ACPs ) could improve substrate shuttling in O8 mutants.
Stress Adaptation: ACP-SpoT interactions modulate (p)ppGpp levels under amino acid starvation , a mechanism exploitable in recombinant systems.

Product Specs

Form

Lyophilized powder. Note: We will preferentially ship the format we have in stock. If you have a specific format requirement, please note it when ordering, and we will fulfill your request.

Lead Time

Delivery times may vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. Note: All proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please contact us in advance, as additional fees will apply.

Notes

Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.

Shelf Life

Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.

Synonyms

acpP; ECIAI1_1129Acyl carrier protein; ACP

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-78

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Escherichia coli O8 (strain IAI1)

Target Names

acpP

Target Protein Sequence

MSTIEERVKK IIGEQLGVKQ EEVTNNASFV EDLGADSLDT VELVMALEEE FDTEIPDEEA EKITTVQAAI DYINGHQA

Uniprot No.

B7LX28

Target Background

Function

This protein functions as the carrier of the growing fatty acid chain during fatty acid biosynthesis.

Database Links

KEGG: ecr:ECIAI1_1129

Protein Families

Acyl carrier protein (ACP) family

Subcellular Location

Cytoplasm.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

Recombinant Escherichia coli O8 Acyl carrier protein (acpP)

Description

Core Function of ACP in E. coli

Key Features of E. coli ACP

O8 Serotype and O-Antigen Biosynthesis

Functional Complementation of E. coli acpP Mutants

Potential Links Between O8 and ACP

Research Gaps and Future Directions

Product Specs

Target Background

Quick Inquiry

Category

Service