Recombinant Escherichia coli O81 ATP synthase subunit alpha (atpA)
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In Stock 
Description
Table 1: Key Properties of Recombinant α-Subunit
Functional Insights
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Assembly prerequisite: Isolated α-subunit does not bind δ-subunit unless complexed with β-subunit, indicating cooperative folding requirements .
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Proton translocation coupling: α-subunit N-terminal residues (1–22) mediate interaction with δ-subunit, essential for stator stability .
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Thermodynamic thresholds: ATP synthesis in E. coli requires a proton motive force (PMF) ≥ 150 mV, influenced by rotor-stator interactions .
Modulation by External Factors
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Lipid interactions: Cardiolipin sequestration by antimicrobial peptides (e.g., EcDBS1R4) disrupts α-subunit interactions with the Fo sector, reducing ATP synthase activity .
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Thermal stability: Disulfide bonds between α-subunit and δ-subunit form optimally at 0–20°C, stabilizing stator assembly .
Operon Context and Evolutionary Notes
Product Specs
Form
Lyophilized powder. We will ship the in-stock format, but if you have special requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. For dry ice shipping, contact us in advance; extra fees apply.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
atpA; ECED1_4424ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-513
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia coli O81 (strain ED1a)
Target Names
atpA
Target Protein Sequence
MQLNSTEISE LIKQRIAQFN VVSEAHNEGT IVSVSDGVIR IHGLADCMQG EMISLPGNRY
AIALNLERDS VGAVVMGPYA DLAEGMKVKC TGRILEVPVG RGLLGRVVNT LGAPIDGKGP
LDHDGFSAVE AIAPGVIERQ SVDQPVQTGY KAVDSMIPIG RGQRELIIGD RQTGKTALAI
DAIINQRDSG IKCIYVAIGQ KASTISNVVR KLEEHGALAN TIVVVATASE SAALQYLAPY
AGCAMGEYFR DRGEDALIIY DDLSKQAVAY RQISLLLRRP PGREAFPGDV FYLHSRLLER
AARVNAEYVE AFTKGEVKGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETNLF
NAGIRPAVNP GISVSRVGGA AQTKIMKKLS GGIRTALAQY RELAAFSQFA SDLDDATRKQ
LDHGQKVTEL LKQKQYAPMS VAQQSLVLFA AERGYLADVE LSKIGSFEAA LLAYVDRDHA
PLMQEINQTG GYNDEIEGKL KGILDSFKAT QSW
Uniprot No.
Target Background
Function
Produces ATP from ADP using a proton gradient across the membrane. The alpha chain is a regulatory subunit.
Database Links
KEGG: ecq:ECED1_4424
Protein Families
ATPase alpha/beta chains family
Subcellular Location
Cell inner membrane; Peripheral membrane protein.
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