Recombinant Escherichia coli O9:H4 Glycogen debranching enzyme (glgX)

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Cat. No.

BT45212

Source

Yeast

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45212

Source

E.coli

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45212

Source

E.coli

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45212

Source

Baculovirus

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45212

Source

Mammalian cell

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Purity

>85% (SDS-PAGE)

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Description

Enzymatic Classification and Functional Role

GlgX (EC 3.2.1.196) is a limit dextrin α-1,6-maltotetraose-hydrolase that selectively hydrolyzes α-1,6-glycosidic linkages in phosphorylase-limit dextrins with branches of three or four glucose residues . Unlike eukaryotic debranching enzymes, GlgX does not require a separate 4-α-glucanotransferase, enabling direct debranching of short outer chains during glycogen breakdown .

Key Reaction

\text{Glycogen phosphorylase-limit dextrin} \xrightarrow{\text{GlgX}} \text{Debranched dextrin} + \text{Maltotriose/maltotetraose}

Genetic Context and Operon Organization

The glgX gene is part of the glgBXCAP operon, which coordinates glycogen synthesis and degradation . Disruption of glgX leads to:

Glycogen overproduction: Mutants (ΔglgX) accumulate glycogen with altered chain-length distributions .
Structural abnormalities: Increased proportions of very short (4–6 DP) and long (≥17 DP) chains compared to wild-type glycogen .

Phenotypic Effects of glgX Deletion

Studies in E. coli K12 mutants reveal:

Growth enhancement: ΔglgX strains show higher OD600 at stationary phase compared to wild-type .
Environmental stress responses:
- Enhanced starvation survival due to glycogen overaccumulation.
- Reduced desiccation tolerance and cold sensitivity .

Research Findings on Recombinant GlgX

While no direct studies on E. coli O9:H4 GlgX exist, recombinant forms are inferred from conserved mechanisms:

**Table 1: Comparative Glycogen Properties in glgX Mutants**

Strain	Glycogen Content (μg/mg)	Average Chain Length (DP)	Phenotype
Wild-type	12.5 ± 1.2	12.04 ± 0.28	Normal growth, iodine+
ΔglgX	28.7 ± 2.1	12.50 ± 0.32	Overproduction, iodine++

Table 2: Enzymatic Properties of GlgX

Parameter	Value	Source
Optimal pH	6.5–7.5
Substrate specificity	3–4 glucose branch chains
KM (maltotetraose)	0.15 mM

Biotechnological Implications

Recombinant GlgX has potential applications in:

Glycogen engineering: Modifying chain-length distributions for industrial polysaccharides.
Metabolic studies: Elucidating glycogen turnover in biofilms and stress responses .

Knowledge Gaps and Future Directions

Strain-specific differences: Functional characterization of O9:H4 GlgX versus K12 is needed.
Structural dynamics: How substrate binding induces conformational changes remains unresolved .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format by default, but special format requests can be made during ordering.

Lead Time

Delivery times vary by purchase method and location. Consult local distributors for specific times. Proteins ship with blue ice packs by default. Dry ice shipping is available upon request for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.

Synonyms

glgX; EcHS_A3631Glycogen debranching enzyme; EC 3.2.1.196; Limit dextrin alpha-1,6-maltotetraose-hydrolase

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-657

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Escherichia coli O9:H4 (strain HS)

Target Names

glgX

Target Protein Sequence

MTQLAIGKPA PLGAHYDGQG VNFTLFSVHA ERVELCVFDA NGQEHRYDLP GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGTLDLVE RRRDSIHALL TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW SQASSGLTAF TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH GLCVFQR

Uniprot No.

A8A5P1

Target Background

Function

Removes maltotriose and maltotetraose chains linked by 1,6-alpha bonds to the limit dextrin main chain, resulting in a debranched limit dextrin.

Database Links

KEGG: ecx:EcHS_A3631

Protein Families

Glycosyl hydrolase 13 family

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Recombinant Escherichia coli O9:H4 Glycogen debranching enzyme (glgX)

Description

Enzymatic Classification and Functional Role

Key Reaction

Genetic Context and Operon Organization

Phenotypic Effects of glgX Deletion

Research Findings on Recombinant GlgX

**Table 1: Comparative Glycogen Properties in glgX Mutants**

Table 2: Enzymatic Properties of GlgX

Biotechnological Implications

Knowledge Gaps and Future Directions

Product Specs

Target Background

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