Recombinant Escherichia coli Protein Ddg (lpxP)
Description
Introduction
Recombinant Escherichia coli Protein Ddg, also known as LpxP, is an acyltransferase enzyme expressed in Escherichia coli . Specifically, LpxP incorporates a palmitoleoyl moiety into nascent lipid A in place of the secondary laurate chain normally added by LpxL(HtrB) . The protein is produced using recombinant DNA technology, where the gene encoding LpxP is inserted into E. coli to facilitate its expression and production .
Role in Cold Adaptation
LpxP is induced by cold shock in E. coli . At low growth temperatures, LpxP incorporates palmitoleate into lipid A molecules . The presence of palmitoleate in lipid A influences the properties of the outer membrane . A mutant lacking LpxP (MKV11) showed a tenfold increase in sensitivity to rifampicin and vancomycin at 12°C compared to wild-type cells, suggesting that palmitoleoyltransferase may confer a selective advantage upon E. coli cells growing at lower temperatures by making the outer membrane a more effective barrier to harmful chemicals .
Applications in Biotechnology
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Lithium Adsorption: Recombinant E. coli can be engineered to selectively adsorb and recover lithium from the environment by employing a bacterial cell surface display strategy . For example, lithium-binding peptides can be integrated into membrane proteins like OmpC to enhance lithium adsorption .
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Protein Production: E. coli is a widely used host for recombinant protein expression due to its rapid growth, well-understood genetics, and ease of genetic manipulation . Various expression systems, such as the T7 promoter system and lambda phage-based systems, are employed for high-level production of heterologous proteins .
Data Table: Properties of Recombinant Escherichia coli Protein Ddg (LpxP)
Product Specs
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Target Background
This recombinant Escherichia coli protein Ddg (lpxP) catalyzes the transfer of palmitoleate from palmitoleoyl-acyl carrier protein (ACP) to Kdo(2)-lipid IV(A), forming Kdo(2)-(palmitoleoyl)-lipid IV(A). It's crucial for the biosynthesis of a specific lipid A molecular species found only in cells grown at low temperatures. This enzyme may provide a selective advantage at lower temperatures by enhancing the outer membrane's barrier function against harmful substances.
KEGG: ecj:JW2375
STRING: 316385.ECDH10B_2543
