Recombinant Escherichia coli Thymidylate kinase (tmk)

Shipped with Ice Packs

In Stock

Cat. No.

BT40661

Source

Yeast

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT40661

Source

E.coli

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT40661

Source

E.coli

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT40661

Source

Baculovirus

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Purity

>85% (SDS-PAGE)

Quick Inquiry

Cat. No.

BT40661

Source

Mammalian cell

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Purity

>85% (SDS-PAGE)

Quick Inquiry

Description

Recombinant Production Systems

In-Frame Deletions: The tmk gene was replaced with the kanamycin resistance gene kka1 to create conditional-lethal strains. These strains require plasmid-encoded tmk for viability, enabling functional complementation studies .
Heterologous Expression: The E. coli tmk gene has been co-expressed with acetate kinase (ACKase) in recombinant E. coli BL21(DE3) for industrial dTTP synthesis .
Complementation Studies: Plasmids expressing thymidylate kinases from bacteriophage T4, yeast, or humans (dTYMK) rescue viability in Δtmk strains, demonstrating cross-species functional compatibility .

Biotechnological Applications

Enzymatic dTTP Synthesis: Recombinant E. coli coexpressing tmk and ACKase achieve >94% conversion of dTMP to dTTP using acetyl phosphate as a phosphate donor .
Nucleoside Analogue Phosphorylation: The enzyme is used to activate antiviral/anticancer nucleoside analogues (e.g., zidovudine, gemcitabine) in therapeutic contexts .
Industrial Scale-Up: Permeabilized cells of recombinant E. coli (strain pTA) retain ~90% enzyme activity after EDTA treatment, enabling efficient continuous production .

Research Findings and Data

Enzymatic Activity:

Enzyme Substrate Yield
E. coli tmk 5 mM dTMP 94% → dTTP
Human dTYMK 5 mM dTMP 85% → dTTP
T4 gp1 kinase 5 mM dTMP 78% → dTTP
Stability: Pretreatment with 20 mM EDTA at 25°C for 30 min preserves 94% tmk activity and 96% ACKase activity in E. coli pTA .

Enzyme	Substrate	Yield
E. coli tmk	5 mM dTMP	94% → dTTP
Human dTYMK	5 mM dTMP	85% → dTTP
T4 gp1 kinase	5 mM dTMP	78% → dTTP

Product Specs

Form

Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipment, please contact us in advance; extra fees apply.

Notes

Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize developing it.

Synonyms

tmk; ECDH10B_1170Thymidylate kinase; EC 2.7.4.9; dTMP kinase

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-213

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Escherichia coli (strain K12 / DH10B)

Target Names

tmk

Target Protein Sequence

MRSKYIVIEG LEGAGKTTAR NVVVETLEQL GIRDMVFTRE PGGTQLAEKL RSLVLDIKSV GDEVITDKAE VLMFYAARVQ LVETVIKPAL ANGTWVIGDR HDLSTQAYQG GGRGIDQHML ATLRDAVLGD FRPDLTLYLD VTPEVGLKRA RARGELDRIE QESFDFFNRT RARYLELAAQ DKSIHTIDAT QPLEAVMDAI RTTVTHWVKE LDA

Uniprot No.

B1XA08

Target Background

Function

Phosphorylates dTMP to dTDP in both de novo and salvage pathways of dTTP synthesis.

Database Links

KEGG: ecd:ECDH10B_1170

Protein Families

Thymidylate kinase family

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Source

Quantity&Size*

Product Name

Message

Recombinant Escherichia coli Thymidylate kinase (tmk)

Description

Recombinant Production Systems

Biotechnological Applications

Research Findings and Data

Product Specs

Target Background

Quick Inquiry

Category

Service