Strain Variations: The O127:H6 strain variant (P58767) exhibits slight sequence divergence (e.g., "FGL" at position 4 vs. "FDL" in K12) .
Membrane Localization: Predicted as a transmembrane protein, aligning with its association with stress response pathways .
System | Details |
---|---|
Host Organism | E. coli (common), cell-free expression (niche applications) |
Expression Region | Full-length (1–84aa) |
Purification | His-tag affinity chromatography |
Application | Description |
---|---|
SDS-PAGE | Quality control and molecular weight confirmation |
ELISA | Antibody binding assays (e.g., epitope mapping) |
Structural Studies | X-ray crystallography or NMR for membrane protein interactions |
Transglycosylase Research | Investigating its role in cell wall synthesis or stress adaptation |
The protein is annotated as a "Transglycosylase-associated gene protein," suggesting a role in peptidoglycan remodeling or cross-linking .
Limited functional data exists; most applications focus on biochemical characterization .
Parameter | Guidelines |
---|---|
Short-Term Storage | -20°C/-80°C (lyophilized) or 4°C (working aliquots, ≤1 week) |
Reconstitution | Deionized water (0.1–1.0 mg/mL) + 5–50% glycerol (for long-term stability) |
Freeze-Thaw Cycles | Avoid repeated cycles to prevent aggregation or degradation |
Supplier | Product Code | Purity | Price (USD/€) | Format |
---|---|---|---|---|
Creative Biomart | RFL13455EF | >90% | Inquiry-based | Lyophilized powder |
Cusabio | CSB-CF301173ENV | Not stated | Not listed | Lyophilized |
MyBioSource | MBS7035194 | ≥85% | $1,345 | Liquid (glycerol) |
CBM15 | CSB-CF301173ENV | Not stated | €1,424 | Lyophilized |
Creative Biomart: Offers strain-specific variants (K12 and O127:H6) .
MyBioSource: Provides partial-length proteins for niche studies .
KEGG: ecj:JW1184
STRING: 316385.ECDH10B_1248