Recombinant Escherichia fergusonii 6-phosphofructokinase (pfkA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will ship the available format, but please specify any format requirements when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs by default. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us and we will prioritize its development.
Synonyms
pfkA; EFER_3857; ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; EC 2.7.1.11; Phosphohexokinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-320
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Target Names
pfkA
Target Protein Sequence
MIKKIGVLTS GGDAPGMNAA IRGVVRAALS EGLEVMGIYD GYLGLYEDRM VQLDRYSVSD
MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV IGGDGSYMGA MRLTEMGFPC
IGLPGTIDND IKGTDYTIGF FTALSTVVEA IDRLRDTSSS HQRISVVEVM GRYCGDLTLA
AAIAGGCEFV VVPEVEFSRE DLVNEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG
RETRATVLGH IQRGGSPVPY DRILASRMGA YAIELLLAGY GGRCVGIQNE QLVHHDIIDA
IENMKRPFKG DWLECAKKLY
Uniprot No.
Target Background
Function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP, the first committed step of glycolysis.
Database Links
KEGG: efe:EFER_3857
Protein Families
Phosphofructokinase type A (PFKA) family, ATP-dependent PFK group I subfamily, Prokaryotic clade "B1" sub-subfamily
Subcellular Location
Cytoplasm.
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