Recombinant Escherichia fergusonii Peptide deformylase (def)
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Description
Introduction to Recombinant Escherichia fergusonii Peptide Deformylase (def)
Recombinant Escherichia fergusonii Peptide Deformylase (def) is a genetically engineered metalloenzyme critical for bacterial protein maturation. It catalyzes the hydrolytic removal of the N-terminal formyl group from nascent polypeptides—a universal step in bacterial protein synthesis . This enzyme is produced via recombinant DNA technology, enabling its study and application in antibacterial drug development .
Expression and Purification
Table 2: Activity Comparison
| Feature | E. fergusonii PDF | Human Mitochondrial PDF |
|---|---|---|
| Catalytic Metal | Fe²⁺/Ni²⁺ | Fe²⁺ (low stability) |
| Deformylation Efficiency | High | Negligible |
| Drug Target Potential | Validated | Not applicable |
Antibacterial Drug Development
-
Target Validation: PDF is essential for bacterial survival; deletion of the def gene in Streptococcus pneumoniae increases susceptibility to deformylase inhibitors .
-
Inhibitor Screening: Recombinant PDF is used to test compounds like actinonin, which block deformylation and cause bacteriostatic effects in E. coli .
Mechanistic Studies
Product Specs
Form
Lyophilized powder. Note: We will preferentially ship the format we have in stock. If you have special format requirements, please specify them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributors for specific delivery times. Note: All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you require a specific tag type, please inform us, and we will prioritize developing it.
Synonyms
def; EFER_3270Peptide deformylase; PDF; EC 3.5.1.88; Polypeptide deformylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-169
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Target Names
def
Target Protein Sequence
MSVLQVLHIP DERLRKVAKP VEEVNAEIQR IVDDMFETMY AEEGIGLAAT QVDIHQRIIV
IDVSENRDER LVLINPELLE KSGETGIEEG CLSIPEQRAL VPRAEKVKIR ALDRDGKPFE
LEADGLLAIC IQHEMDHLVG KLFMDYLSPL KQQRIRQKVE KLDRLKARA
Uniprot No.
Target Background
Function
Removes the formyl group from the N-terminal methionine of newly synthesized proteins. Requires at least a dipeptide for efficient activity. N-terminal L-methionine is essential for activity, but the enzyme has broad specificity at other positions.
Database Links
KEGG: efe:EFER_3270
Protein Families
Polypeptide deformylase family
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