Recombinant Extracellular superoxide dismutase [Cu-Zn] (sod-4)

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Cat. No.

BT35056

Source

Yeast

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35056

Source

E.coli

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35056

Source

E.coli

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35056

Source

Baculovirus

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35056

Source

Mammalian cell

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Purity

>85% (SDS-PAGE)

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Description

Functional Roles in Pathogenesis

SOD4 is critical for fungal virulence, particularly under iron-limited host conditions:

Iron Starvation Response: Transcriptionally regulated by the iron-responsive factor SEF1, SOD4 is upregulated during iron deprivation to mitigate oxidative stress .
Hyphal Morphogenesis: While SOD5 is induced during hyphal growth, SOD4 expression remains independent of morphological changes, instead focusing on iron homeostasis .
Host Immune Evasion: By neutralizing extracellular superoxide, SOD4 protects fungal cells from oxidative bursts generated by immune cells .

Expression Systems

Prokaryotic Systems: E. coli yields SOD4 with $V_{\text{max}} = 38.73 \, \text{mmol/min}$ and $K_m = 0.07 \, \text{mmol/L}$ , comparable to eukaryotic systems like Pichia pastoris .
Post-Translational Modifications: Unlike native SOD4, recombinant versions lack glycosylation, affecting thermal stability (half-life of 15 minutes at 90°C) .

Therapeutic Potential

Recombinant SOD4 has been explored in gene therapy models for cardiovascular and neurological protection:

Myocardial Stunning: Adenovirus-mediated delivery of SOD4 reduced infarct size by 50% in rabbits by targeting extracellular superoxide at the sarcolemma .
Neuroprotection: Intracranial gene transfer in rabbits increased cerebrospinal fluid SOD activity by 148 U/mL, enhancing resistance to ischemic stroke .

Regulatory Mechanisms

SOD4 activity is tightly controlled by environmental cues:

Transcriptional Regulation:
- Induced by iron starvation via SEF1 .
- Unaffected by hyphal differentiation signals (unlike SOD5) .
Post-Translational Modulation:
- Heparin displaces SOD4 from heparan sulfate proteoglycans, redistributing it to tissues .

Comparative Analysis with Other SODs

Parameter	SOD4	EC-SOD (SOD3)	Cu/Zn-SOD (SOD1)
Localization	Cell wall/extracellular	Extracellular matrix	Cytoplasm
Metal Dependency	Cu-only	Cu/Zn	Cu/Zn
Pathogen Association	Candida virulence	Mammalian antioxidant defense	Ubiquitous cellular defense
Therapeutic Half-Life	20 hours (plasma)	6–8 hours (plasma)	7 minutes (plasma)

Challenges and Future Directions

Catalytic Limitations: The absence of zinc reduces structural stability, necessitating protein engineering for clinical applications .
Host Specificity: SOD4’s fungal origin may trigger immune responses in mammals, requiring humanized variants for therapeutics .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering, and we will accommodate your request.

Lead Time

Delivery times vary depending on the purchase method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance, as additional charges will apply.

Notes

Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.

Shelf Life

Shelf life depends on several factors including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, while the lyophilized form is stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during the manufacturing process. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

sod-4; F55H2.1; Extracellular superoxide dismutase [Cu-Zn]; EC-SOD; EC 1.15.1.1

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

20-221

Protein Length

Extracellular domain

Purity

>85% (SDS-PAGE)

Species

Caenorhabditis elegans

Target Names

sod-4

Target Protein Sequence

E VIRARAYIFK AEAGKIPTEL IGTIDFDQSG SFLKLNGSVS GLAAGKHGFH IHEKGDTGNG CLSAGGHYNP HKLSHGAPDD SNRHIGDLGN IESPASGDTL ISVSDSLASL SGQYSIIGRS VVIHEKTDDL GRGTSDQSKT TGNAGSRLAC GTIGTVEERI LETTTASLPP VTQSQPIGSS SYYYSTFYLP IILYFLLSRI L

Uniprot No.

P34461

Target Background

Function

Neutralizes radicals normally produced within cells that are toxic to biological systems.

Database Links

STRING: 6239.F55H2.1b

UniGene: Cel.19659

Protein Families

Cu-Zn superoxide dismutase family

Subcellular Location

[Isoform 1]: Secreted, extracellular space.; [Isoform 2]: Membrane; Peripheral membrane protein.

Tissue Specificity

Isoform 2 is preferentially expressed in eggs.

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Recombinant Extracellular superoxide dismutase [Cu-Zn] (sod-4)

Description

Functional Roles in Pathogenesis

Expression Systems

Therapeutic Potential

Regulatory Mechanisms

Comparative Analysis with Other SODs

Challenges and Future Directions

Product Specs

Target Background

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