Recombinant General secretion pathway protein B (exeB)
Description
Introduction to Recombinant General Secretion Pathway Protein B (ExeB)
ExeB is an inner membrane protein involved in the type II secretion pathway in Gram-negative bacteria . This pathway is essential for the translocation of proteins across both the cytoplasmic and outer membranes in a two-step process . The secretion of toxins and other virulence factors relies on the ExeAB complex, where ExeB partners with ExeA to facilitate this process .
Interaction with ExeA
The ExeA protein contains a consensus ATP-binding site, and mutations within this site significantly reduce the rate of toxin secretion . The interaction between ExeA and ExeB is critical for the function of the type II secretion system .
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ATP Binding: ExeA likely uses ATP to provide energy for the secretion process, suggesting that ATP binding by ExeA is required for ExeA-ExeB complex formation .
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Hydrolysis: Hydrolysis is required for its function in secretion once established .
Role in Protein Secretion
ExeB, in conjunction with ExeA, plays a crucial role in protein secretion . The ExeAB complex is essential for the energy-dependent secretion of proteins like aerolysin in Aeromonas hydrophila .
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Secretion Mechanism: The ExeAB complex likely transduces metabolic energy to facilitate the opening of a secretion port in the outer membrane, enabling the translocation of proteins .
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Requirement for ExeA: ExeB's function is heavily reliant on its interaction with ExeA, as evidenced by the instability of ExeB in the absence of ExeA .
Product Specs
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Target Background
Involved in the general secretion pathway (GSP) for protein export.
