Recombinant Geobacillus sp. Phosphatidylserine decarboxylase proenzyme (psd)

Shipped with Ice Packs
In Stock
Cat. No.
BT42088
Source
Yeast
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42088
Source
E.coli
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42088
Source
E.coli
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42088
Source
Baculovirus
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT42088
Source
Mammalian cell
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Recombinant Expression and Purification

Recombinant PSD production typically employs Escherichia coli expression systems:

  • Host strain: E. coli C41 (DE3) is preferred for soluble expression of thermophilic enzymes, as demonstrated for Geobacillus lipases .

  • Tagging: Fusion tags (e.g., 6xHis-SUMO) facilitate purification via immobilized metal affinity chromatography (IMAC) .

  • Purification workflow:

    StepYield (%)Purity (%)Method Details
    Heat treatment5060–7060°C for 30–60 min
    Cation exchange2190NaCl gradient elution
    Size-exclusion chromatography18>95Molecular mass validation

Catalytic Mechanism

  • Substrate specificity: Binds PS via ionic interactions (Kₐ = ~80 nM for Plasmodium PSD) .

  • Regulation: Maturation is activated by anionic lipids (e.g., PS) and inhibited by phosphatidylglycerol (PG) or phosphatidic acid (PA) .

Enzyme Kinetics

While kinetic parameters for Geobacillus PSD are not explicitly reported, analogous bacterial PSDs exhibit:

ParameterValue (Homologous Enzyme)Conditions
Optimal pH7.5–8.5Mitochondrial membrane
Temperature stability>60°CThermostable variants
Vₘₐₓ0.8–1.2 µmol/min/mgPS substrate

Biotechnological Applications

  • Membrane biogenesis: Essential for PE synthesis in synthetic biology applications .

  • Industrial catalysis: Potential use in lipid remodeling due to thermostability and solvent tolerance .

Research Challenges

  • Low soluble expression: Addressed by strain optimization (e.g., E. coli C41 over BL21) .

  • Autoinhibition: Lipid-mediated regulation necessitates precise in vitro conditions for maturation .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format, but if you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies based on purchasing method and location. Consult your local distributor for specifics. All proteins ship with blue ice packs by default. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize its development.
Synonyms
psd; GWCH70_2462; Phosphatidylserine decarboxylase proenzyme; EC 4.1.1.65) [Cleaved into: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain]
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-225
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Geobacillus sp. (strain WCH70)
Target Names
psd
Target Protein Sequence
MLKWLYRLMI ELTNHSLSSK MIAIFTKSRL SALLIPSYAK IYDINQEEME KNLKNYETLQ QLFIRKLKAG TRPIDQTKNS VISPVDAIIE DIGVIRENSE IVVKGKTYSI AEMLGSDEAA KKYLNGLFII LYLSPSHYHR IHSPISGVVQ KQWALGKKSY PVNRLGLKYG KRPLSKNYRM ITEVAANGKH IAIVKIGAMF VNSIELTHAS EHLTKGQEIA YFSFG
Uniprot No.
C5D4W6

Target Background

Function
Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).
Database Links

KEGG: gwc:GWCH70_2462

STRING: 471223.GWCH70_2462

Protein Families
Phosphatidylserine decarboxylase family, PSD-B subfamily, Prokaryotic type I sub-subfamily
Subcellular Location
Cell membrane; Peripheral membrane protein.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.