Recombinant Geobacillus stearothermophilus 23 kDa basic protein
Description
Overview of Recombinant Proteins from G. stearothermophilus
G. stearothermophilus is renowned for thermostable enzymes, including α-amylases, esterases, β-galactosidases, and S-layer proteins. Recombinant expression systems (e.g., Pichia pastoris, Escherichia coli) are frequently employed to enhance yield and stability . Key features of these proteins include:
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pH stability: Optimal activity typically between pH 6.0–8.0 .
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Industrial relevance: Applications in starch processing, biofilm removal, and oligosaccharide synthesis .
Hypothetical Profile of a 23 kDa Basic Protein
While no literature explicitly describes a 23 kDa basic protein, extrapolation from homologous systems suggests potential attributes:
Biochemical Properties
Functional Domains
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Basic residues: High lysine/arginine content could facilitate interactions with negatively charged substrates (e.g., DNA, cell wall polymers) .
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Potential roles: Catalytic activity (e.g., hydrolase), structural function (e.g., S-layer anchoring), or antimicrobial activity .
Expression and Purification Strategies
Recombinant proteins from G. stearothermophilus are often expressed in heterologous systems:
Case Study: α-Amylase SR74
Example Protocol
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Gene amplification: PCR-cloned with methanol-inducible promoter (AOX) .
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Characterization: SDS-PAGE, activity assays, thermal/pH stability tests .
