Recombinant Geobacter lovleyi ATP synthase subunit c (atpE)
Description
Production and Purification
The recombinant atpE is manufactured via bacterial expression systems, with rigorous quality control:
| Parameter | Detail |
|---|---|
| Expression Host | E. coli |
| Purity | >90% (SDS-PAGE) |
| Storage Buffer | Tris/PBS-based buffer with 6% trehalose (pH 8.0) |
| Reconstitution | Dissolved in sterile water (0.1–1.0 mg/mL) with 5–50% glycerol for stability |
Freeze-thaw cycles are avoided to prevent degradation, and working aliquots are stored at 4°C for ≤1 week .
Functional Role and Biotechnological Relevance
The atpE subunit is a core component of the F₀ sector in ATP synthase, enabling proton translocation across membranes. In Geobacter lovleyi, this enzyme supports energy conservation during organohalide respiration, such as PCE-to-cis-DCE dechlorination .
Applications in Research
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Biochemical Assays: Used to study proton-coupled ATP synthesis and subunit interactions .
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ELISA Development: Serves as an antigen for detecting anti-atpE antibodies or validating assay specificity .
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Structural Studies: His-tag facilitates crystallography or cryo-EM to resolve c-ring assembly in ATP synthase .
Comparative Genomic and Functional Insights
Geobacter lovleyi diverges from other Geobacter species in its genomic adaptations for organohalide respiration. For example:
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Genomic Islands: The pce gene cluster (encoding reductive dehalogenases) is absent in non-PCE-respiring Geobacter strains .
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Cobalamin Biosynthesis: Plasmid pSZ77 in G. lovleyi strain SZ encodes 15/24 enzymes for cobalamin synthesis, a cofactor essential for organohalide respiration .
| Feature | G. lovleyi | Other Geobacter spp. |
|---|---|---|
| PCE Respiration | Enabled by pce-genes | Absent |
| Cobalamin Genes | Plasmid-encoded (pSZ77) | Chromosomal |
| Cytochrome Content | Reduced c-type cytochromes | Higher abundance |
Research Gaps and Future Directions
While the recombinant atpE is well-characterized for structural studies, its role in G. lovleyi’s unique metabolic pathways (e.g., Na⁺ vs. H⁺ transport) remains underexplored. Future work could:
Product Specs
Note: We prioritize shipping the format readily available in our inventory. However, if you have specific format requirements, please indicate them when placing your order. We will fulfill your request to the best of our ability.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: glo:Glov_3143
STRING: 398767.Glov_3143
