Recombinant Geobacter sulfurreducens Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial
Description
Definition and Functional Role
Recombinant Geobacter sulfurreducens Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (GlmS) is a partially purified enzyme produced through genetic engineering. GlmS catalyzes the first committed step in hexosamine biosynthesis, converting fructose-6-phosphate and glutamine into glucosamine-6-phosphate and glutamate . This pathway is critical for bacterial cell wall synthesis and amino sugar metabolism. The "partial" designation indicates that the recombinant protein represents a truncated form of the full-length enzyme, likely retaining catalytic activity but lacking certain domains .
Gene and Host Systems
-
Host Systems: Expressed in E. coli, yeast, baculovirus, or mammalian cells, with ≥85% purity confirmed via SDS-PAGE .
Key Applications
-
Metabolic Studies: Used to investigate amino sugar biosynthesis in G. sulfurreducens, a model organism for extracellular electron transfer and bioremediation .
-
Biotechnological Relevance: Supports studies on bacterial survival strategies under stress (e.g., oxidative or metal-reducing conditions) .
Production Metrics
| Property | Detail | Source |
|---|---|---|
| Host | E. coli, yeast, baculovirus | |
| Purity | ≥85% (SDS-PAGE) | |
| Catalytic Activity | Retains isomerizing function | Inferred |
| Gene Identifier | glmS |
Link to Extracellular Electron Transfer (EET)
While GlmS is not directly involved in EET, its role in cell wall synthesis supports biofilm formation, a critical process for G. sulfurreducens during:
Transcriptional Regulation
-
GlmS expression may be modulated under microaerobic conditions, where G. sulfurreducens upregulates biofilm-related genes (e.g., pilA-N, omcZ) to mitigate oxidative stress .
-
Feedback inhibition by glucosamine-6-phosphate (GlcN-6-P) is a conserved regulatory mechanism observed in E. coli , likely applicable to G. sulfurreducens.
Comparative Analysis with Homologs
Research Gaps and Future Directions
-
Structural Insights: High-resolution crystallography is needed to resolve the active site and regulatory regions.
-
In Vivo Functionality: No direct studies confirm GlmS’s role in G. sulfurreducens biofilms or EET pathways.
-
Industrial Potential: Engineered variants could optimize amino sugar production for biomanufacturing .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize its development.
Target Background
This protein catalyzes the initial step in hexosamine metabolism, converting fructose-6-phosphate to glucosamine-6-phosphate using glutamine as a nitrogen source.
KEGG: gsu:GSU0270
STRING: 243231.GSU0270
