Recombinant Gloeobacter violaceus Anhydro-N-acetylmuramic acid kinase (anmK)
Description
Enzymatic Function and Biological Role
AnmK belongs to the peptidoglycan recycling pathway, which salvages up to 60% of cell wall components in bacteria like Escherichia coli . In Gloeobacter violaceus, AnmK phosphorylates anhMurNAc, enabling its conversion to N-acetylglucosamine-phosphate (GlcNAc-P) via subsequent enzymatic steps. This process ensures the reuse of cell wall sugars, minimizing energy expenditure on de novo synthesis .
Key reactions:
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Substrate: 1,6-anhydro-N-acetylmuramic acid (anhMurNAc)
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Product: N-acetylmuramic acid-6-phosphate (MurNAc-P)
Genetic and Evolutionary Context
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Genomic Localization: The anmK gene in Gloeobacter violaceus (UniProt Q7NEY1) is part of a conserved operon linked to cell wall recycling .
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Phylogenetic Significance: Gloeobacter represents an early-branching cyanobacterium lacking thylakoid membranes, making its enzymes valuable for studying ancestral metabolic pathways .
Applications in Research
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Antibiotic Susceptibility Studies: Disruption of anmK in P. aeruginosa increases sensitivity to β-lactams like imipenem, highlighting its role in cell wall integrity .
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Enzyme Engineering: Recombinant AnmK enables high-sensitivity detection of peptidoglycan fragments via radioactive labeling .
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Evolutionary Biology: Comparative studies with Gloeobacter AnmK shed light on the adaptation of peptidoglycan recycling in early-diverging bacteria .
Challenges and Future Directions
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will accommodate your request.
Note: All proteins are shipped with standard blue ice packs by default. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: gvi:glr3746
STRING: 251221.glr3746
