Recombinant Gloeobacter violaceus ATP synthase subunit a (atpB)
Description
Molecular Definition and Functional Role
The atpB gene encodes subunit a, a hydrophobic protein essential for forming the proton channel in the F₀ sector of ATP synthase. In Gloeobacter violaceus (strain PCC 7421), this subunit facilitates proton movement across the membrane, driving the rotation of the F₁ sector and enabling ATP synthesis . The recombinant version is expressed in E. coli with an N-terminal His tag (Q7NCR8) to enhance solubility and purification efficiency .
Key Features:
| Parameter | Details | Source |
|---|---|---|
| UniProt ID | Q7NCR8 | |
| AA Sequence (1-246) | MELALATLGNQPLIAAVEVGKHLTWQLGPLSVHGQTMITTWVVmLLLIGLTFIGTRKLQR... | |
| Molecular Weight | ~27.5 kDa (predicted) | |
| Gene Locus | gll2910 |
Production and Purification
The recombinant protein is produced via heterologous expression in E. coli, leveraging its simplicity for large-scale production.
Production Specifications:
| Parameter | Details | Source |
|---|---|---|
| Host Organism | E. coli | |
| Tag | N-terminal His tag | |
| Storage Buffer | Tris-based buffer, 50% glycerol | |
| Storage Conditions | -20°C (long-term), 4°C (short-term) |
Applications:
-
Biochemical Assays: ATP hydrolysis/synthesis activity measurements .
-
ELISA: Antigen in immunoassays for studying ATP synthase regulation .
Research Findings and Mechanistic Studies
Subunit a’s role in proton translocation is central to ATP synthase function. Studies in related cyanobacteria (e.g., Synechocystis PCC 6803) reveal that ATP synthase activity is regulated by environmental cues:
| Condition | Impact on ATP Synthase Activity | Source |
|---|---|---|
| Darkness | Induces AtpΘ expression to inhibit ATP hydrolysis . | |
| Proton Gradient | Sustains ATP synthesis; collapse triggers AtpΘ-mediated inhibition |
Functional Studies:
-
Knockout Experiments: Deletion of atpT (encoding AtpΘ) abolishes darkness-induced inhibition of ATPase activity .
-
Purified ATP Synthase: Recombinant subunit a, when incorporated into purified complexes, enables direct measurement of proton-driven ATP synthesis .
Interaction Partners and Regulatory Networks
Subunit a interacts with multiple components of the ATP synthase complex, as identified through co-immunoprecipitation and far-Western blotting .
Interaction Partners:
Product Specs
Note: We will prioritize shipping the format we currently have in stock. However, if you have specific format requirements, please include them in your order notes, and we will accommodate your request.
Note: All protein orders are shipped with standard blue ice packs. If dry ice shipping is required, please notify us in advance as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form maintains a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: gvi:gvip403
STRING: 251221.gvip403
