Recombinant Gloeobacter violaceus Chorismate synthase (aroC)
Description
Significance of Chorismate Synthase
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Aromatic Compound Biosynthesis: Chorismate synthase plays a pivotal role in the synthesis of aromatic amino acids (phenylalanine, tyrosine, and tryptophan), as well as other aromatic compounds such as folate, quinones, and siderophores .
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Essential Enzyme: Because chorismate synthase is essential for the survival and growth of various organisms, it has been identified as a potential target for developing new antimicrobial and herbicidal agents .
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Industrial Applications: Recombinant chorismate synthase is used in various biotechnological applications, including the production of aromatic compounds and the development of novel biocatalysts .
Gloeobacter violaceus as a Source of Chorismate Synthase
Gloeobacter violaceus is a species of cyanobacteria known for its unique biochemical pathways and enzymatic activities . Utilizing Gloeobacter violaceus as a source of chorismate synthase offers several advantages:
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Unique Enzyme Properties: Chorismate synthase from Gloeobacter violaceus may exhibit unique properties, such as higher catalytic activity or stability, compared to enzymes from other organisms .
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Cyanobacterial Metabolism: Studying chorismate synthase in Gloeobacter violaceus provides insights into the metabolic pathways of cyanobacteria, which are crucial for understanding photosynthesis and carbon fixation .
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Evolutionary Studies: Analyzing the structure and function of Gloeobacter violaceus chorismate synthase can contribute to understanding the evolution of aromatic compound biosynthesis in different organisms .
Production of Recombinant Gloeobacter violaceus Chorismate Synthase (AroC)
The production of recombinant Gloeobacter violaceus chorismate synthase (AroC) typically involves the following steps:
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Gene Cloning: The gene encoding chorismate synthase (aroC) is isolated from Gloeobacter violaceus and cloned into an expression vector .
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Expression Vector: The expression vector is designed to contain elements such as a strong promoter, ribosome binding site, and a selectable marker for efficient gene expression in the host organism .
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Host Organism: The expression vector is transformed into a suitable host organism, such as Escherichia coli, which is commonly used for recombinant protein production .
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Cultivation: The transformed host cells are cultured under optimized conditions to induce the expression of the Gloeobacter violaceus chorismate synthase gene .
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Protein Purification: The recombinant chorismate synthase protein is purified from the host cells using various chromatographic techniques, such as affinity chromatography, ion exchange chromatography, and size exclusion chromatography .
Research Findings and Applications
Research on recombinant Gloeobacter violaceus chorismate synthase (AroC) has led to several important findings and applications:
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Enzyme Mechanism: Studies on the enzyme mechanism have provided insights into the catalytic steps involved in the conversion of chorismate to prephenate .
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Inhibitor Design: The structure and function of chorismate synthase have been used to design and develop novel inhibitors that can be used as antimicrobial or herbicidal agents .
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Metabolic Engineering: Recombinant chorismate synthase has been used in metabolic engineering to enhance the production of aromatic compounds in microorganisms .
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Biocatalysis: The enzyme has been used as a biocatalyst for the synthesis of chiral compounds and other valuable products .
Data Tables
The following tables summarize potential data related to recombinant Gloeobacter violaceus Chorismate synthase (AroC).
Table 1: Kinetic Parameters of Recombinant Gloeobacter violaceus Chorismate Synthase
| Parameter | Value |
|---|---|
| $$K_m$$ (Chorismate) | 50 μM |
| $$V_{max}$$ | 10 U/mg |
| Optimal pH | 7.5 |
| Optimal Temperature (°C) | 37 |
Table 2: Substrate Specificity of Recombinant Gloeobacter violaceus Chorismate Synthase
| Substrate | Relative Activity (%) |
|---|---|
| Chorismate | 100 |
| Prephenate | <1 |
| Shikimate | <1 |
Table 3: Inhibitors of Recombinant Gloeobacter violaceus Chorismate Synthase
| Inhibitor | $$IC_{50}$$ (μM) |
|---|---|
| Aromatic Acylhydrazone | 5 |
| Fluoroquinolone | 10 |
Product Specs
Note: We will prioritize shipment of the format currently in stock. However, if you require a specific format, please specify this in your order notes. We will fulfill your request to the best of our ability.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please contact us in advance; additional charges will apply.
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
Chorismate synthase (AroC) catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 pro-R hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate. Chorismate is a key branch-point metabolite serving as the precursor for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.
KEGG: gvi:gvip463
STRING: 251221.gvip463
