Recombinant Gluconobacter oxydans 50S ribosomal protein L23 (rplW)
Description
Production and Purification
Recombinant rplW is typically expressed in heterologous systems for functional studies. Key production parameters include:
Note: Purification protocols often include reconstitution in deionized water with glycerol (5–50% final concentration) to enhance stability .
Role in Translation and Regulation
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Ribosome assembly: L23 is essential for the structural integrity of the 50S subunit, ensuring proper tRNA accommodation and peptide bond formation .
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Autogenous regulation: In E. coli, L23 homologs regulate their own gene expression via interactions with mRNA leader sequences . While direct evidence for G. oxydans rplW is lacking, analogous mechanisms may exist.
Moonlighting Functions
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Stress response: In mammalian systems, L23 interacts with MDM2 to regulate p53 activity, highlighting potential roles in cellular stress responses . This suggests G. oxydans rplW could modulate stress pathways, particularly under oxidative or nutrient-limited conditions.
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Metabolic interactions: Ribosomal proteins in G. oxydans often associate with membrane-bound dehydrogenases, hinting at indirect roles in substrate oxidation .
Applications in Biotechnology
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Industrial strain optimization: Engineering G. oxydans strains with modified rplW could enhance growth rates or substrate tolerance, as seen in mutants lacking gluconate dehydrogenases .
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Biocatalysis: Recombinant rplW may serve as a scaffold for designing novel enzymes or improving existing biocatalytic pathways .
Research Gaps and Future Directions
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Structural characterization: Crystallographic or cryo-EM studies of G. oxydans rplW are needed to elucidate its interactions with RNA or other ribosomal proteins.
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Functional validation: Direct evidence for autogenous regulation or moonlighting roles in G. oxydans remains sparse.
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Industrial relevance: Exploring rplW’s impact on biofilm formation or stress resistance could enhance biotechnological applications .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us for preferential development.
Target Background
Recombinant Gluconobacter oxydans 50S ribosomal protein L23 (rplW) is an early assembly protein that binds 23S rRNA. It is located near the polypeptide exit tunnel on the ribosome's exterior, forming the primary docking site for trigger factor binding to the ribosome.
KEGG: gox:GOX0378
STRING: 290633.GOX0378
