Recombinant Gluconobacter oxydans tRNA-specific 2-thiouridylase mnmA (mnmA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless otherwise requested. Dry ice shipping requires prior arrangement and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a reference.
Shelf Life
Shelf life depends on various factors, including storage conditions, buffer composition, temperature, and the protein's inherent stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Upon receipt, store at -20°C/-80°C. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
mnmA; GOX1371; tRNA-specific 2-thiouridylase MnmA; EC 2.8.1.13
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-361
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans)
Target Names
mnmA
Target Protein Sequence
MRILVAMSGG VDSSVVAALL KRQGHEVIGA TLQLYDHGQA AKPGACCAGR DIMDARAVAD
RLGFPHYVID AESRFRDSVV ESFADAYARG ETPVPCVACN QGVKFTDLLG MARDLGCEAM
ATGHYVRRVE GPEGAEMHRP VDAERDQTWF LFATTKDQLD YLRFPLGEMP DKAHVRALAQ
ELGLEIAAKP DSQDICFVPS GSYAELVEKL RPEVRGEGEI VDEDGRVLGR HEGVARYTVG
QSKRLGDIHT ATGERQMVTR IDVPKRRIVV GPRVQVSEAD SRRSVRLRDM NWLIDAPAEG
VRCGVQIRAR EKLREALVKP LENGGALVEL TEAAMPAPGQ ACVLYDGSRV LGGGFITAGD
A
Uniprot No.
Target Background
Function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, resulting in the formation of s2U34.
Database Links
KEGG: gox:GOX1371
STRING: 290633.GOX1371
Protein Families
MnmA/TRMU family
Subcellular Location
Cytoplasm.
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