Recombinant Glutamine synthetase 2 isozyme

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Cat. No.
BT56171
Source
Yeast
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56171
Source
E.coli
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56171
Source
E.coli
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56171
Source
Baculovirus
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Purity
>85% (SDS-PAGE)
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Cat. No.
BT56171
Source
Mammalian cell
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Purity
>85% (SDS-PAGE)
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Description

Definition and Overview

Recombinant GS2 isozyme refers to the chloroplastic or organelle-specific glutamine synthetase variant produced via heterologous expression systems (e.g., Escherichia coli, CHO cells). Unlike cytosolic GS1 isoforms, GS2 is primarily involved in assimilating ammonium derived from photorespiration and nitrate reduction in plants . In bacteria and mammals, GS2 homologs regulate nitrogen fixation and neurotransmitter recycling, respectively .

Engineering Insights

  • Mutant S54A: A marine bacterial GS2 variant with 10.7x higher cold adaptability due to enhanced active-site flexibility .

  • R4D Mutation: Disrupts oligomerization in D. melanogaster GS2, abolishing activity .

Catalytic Efficiency

  • Plant GS2: TaGS2 in wheat embryos contributes 40–60% of total GS activity during seed development .

  • Human GS2: Exhibits dual functionality as a glutamine synthetase and palmitoyltransferase for RHOJ .

Regulatory Mechanisms

  • Reversible Adenylylation: R. palustris GlnA1 activity is modulated by GlnE to balance NH₄⁺ assimilation and nitrogenase expression .

  • Feedback Inhibition: H. pylori GS is inhibited by glycine and alanine, optimizing NH₃ utilization .

Applications

  • Bioremediation: Engineered GS2 variants enhance NH₃ removal in wastewater treatment .

  • Agriculture: Overexpression of GS2 in crops improves nitrogen-use efficiency and yield .

  • Biopharmaceuticals: GS-knockout CHO systems enable high-yield antibody production with reduced metabolic waste .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have specific format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies based on purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Contact us in advance if you require dry ice shipping, and additional fees will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, and the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during production. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
Glutamine synthetase 2 isozyme; EC 6.3.1.2; Chloroplast GS2; Glutamate--ammonia ligase; Fragment
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-25
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Emiliania huxleyi (Pontosphaera huxleyi)
Target Protein Sequence
XIAGSEEYGK HCFKGAVADA YLAKH
Uniprot No.
P81643

Target Background

Function
Plays a key role in nitrogen metabolism in microorganisms, animals, and plants.
Protein Families
Glutamine synthetase family
Subcellular Location
Plastid, chloroplast.

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