Recombinant Gorilla gorilla gorilla Suppressor of tumorigenicity 7 protein (ST7)
Description
Overview of Recombinant Gorilla gorilla gorilla Suppressor of Tumorigenicity 7 Protein (ST7)
Recombinant Gorilla gorilla gorilla Suppressor of Tumorigenicity 7 protein (ST7) is a manufactured version of the ST7 protein found in the Western lowland gorilla (Gorilla gorilla gorilla) . ST7, also known as LRP12, RAY1, TSG7, and FAM4A1, is a type I transmembrane protein that belongs to the LDLR superfamily . It is similar in structure to ST7 proteins found in other species, sharing 98% amino acid sequence identity within the extracellular domain with bovine, equine, and porcine ST7 .
Key Features:
Protein Structure and Characteristics
The ST7 protein has multiple structural features :
-
A 32 amino acid signal sequence.
-
A 460 amino acid extracellular domain (ECD) containing two CUB domains and five LDLR class A domains.
-
A 21 amino acid transmembrane domain.
-
A 346 amino acid cytoplasmic domain containing motifs implicated in endocytosis and signal transduction.
Gene Information
The gene encoding ST7 is referred to as ST7 . Genomic sequencing suggests the potential for up to 18 splicing isoforms, though their expression has not been thoroughly investigated .
Expression and Tissue Distribution
ST7 is broadly expressed in normal tissues, particularly in fibroblasts . The highest mRNA levels have been found in the heart and skeletal muscle .
Recombinant Protein Properties
Recombinant ST7 protein is available as a lyophilized powder . It is recommended to reconstitute the protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL, with the possible addition of 5-50% glycerol for long-term storage at -20°C/-80°C . Repeated freezing and thawing should be avoided .
Applications
Recombinant human ST7 protein can be used in various bioactivity assays . When immobilized at 0.5 µg/mL, the concentration of recombinant human LRPAP (Catalog # 4296-LR) that produces 50% of the optimal binding response is approximately 0.6‑3 μg/mL .
Data Table
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The specific tag type is determined during production. If you require a particular tag, please specify it in your order; we will prioritize its use.
Target Background
STRING: 9593.ENSGGOP00000014059
Q&A
What are the optimal storage and handling conditions for recombinant Gorilla gorilla gorilla ST7 protein?
Recombinant Gorilla gorilla gorilla ST7 protein is typically supplied as a lyophilized powder and requires specific handling protocols to maintain stability and activity :
| Storage Parameter | Recommended Condition |
|---|---|
| Long-term storage | -20°C to -80°C |
| Working aliquots | 4°C for up to one week |
| Storage buffer | Tris/PBS-based buffer, 6% Trehalose, pH 8.0 |
| Reconstitution | Deionized sterile water to 0.1-1.0 mg/mL |
| Recommended additives | 5-50% glycerol (final concentration) |
| Freeze-thaw cycles | Minimize; repeated cycles not recommended |
Methodological considerations:
-
Prior to opening, briefly centrifuge the vial to bring contents to the bottom
-
Reconstitute in deionized sterile water to the recommended concentration
-
For long-term storage, aliquot the reconstituted protein with glycerol and store at -20°C/-80°C
-
Avoid repeated freeze-thaw cycles as they can lead to protein degradation
-
When preparing working solutions, maintain sterile conditions to prevent microbial contamination
How can researchers effectively express and purify recombinant Gorilla gorilla gorilla ST7 protein for functional studies?
Expression and purification of recombinant Gorilla gorilla gorilla ST7 protein involves several critical methodological steps:
-
Expression System Selection: E. coli is the most commonly used expression system for Gorilla ST7 protein . BL21(DE3) or Rosetta strains are recommended for optimal expression.
-
Vector Design:
-
Insert the full-length Gorilla ST7 coding sequence (1-585 amino acids) into an expression vector
-
Include an N-terminal His-tag for purification purposes
-
Optimize codon usage for E. coli to enhance expression efficiency
-
-
Purification Strategy:
-
Quality Control:
-
Verify protein identity by Western blot or mass spectrometry
-
Assess protein folding by circular dichroism
-
Test biological activity through functional assays
-
What are the molecular mechanisms through which ST7 exerts its tumor suppressor activity, and how can recombinant Gorilla ST7 protein be used to investigate these pathways?
The molecular mechanisms of ST7-mediated tumor suppression are complex and still being elucidated. Research suggests several key pathways:
-
Regulation of Angiogenesis:
-
Cell Cycle Regulation:
-
Interaction with Other Tumor Suppressors:
-
Epigenetic Regulation:
Experimental approaches using recombinant Gorilla ST7 protein:
