Recombinant Gracilaria tenuistipitata var. liui 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH)
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Description
Introduction
Gracilaria tenuistipitata var. liui 3-oxoacyl-[acyl-carrier-protein] synthase 3, commonly known as FabH, is a condensing enzyme that initiates fatty acid biosynthesis in bacteria. It is involved in the type II fatty acid (FA) biosynthesis pathway, which is responsible for synthesizing fatty acids . FabH produces acetoacetyl-acyl carrier protein (ACP), which is a precursor for the elongation cycle where two carbons are added to the acyl-ACP intermediate until the fatty acid reaches the appropriate length .
Role in Fatty Acid Composition
The following table summarizes the changes in fatty acid composition observed in fabH mutants:
| Fatty Acid | Change in fabH Mutants |
|---|---|
| Unsaturated FA | Increase |
| C16:0 | Decrease |
| C18:1ω7c | Increase |
| C16:1ω7c | Decrease |
Genetic Variation in Gracilaria tenuistipitata var. liui
Genetic analysis of Gracilaria tenuistipitata var. liui has revealed genetic variation among different geographical locations . Microsatellite markers and cox1 gene analysis have been used to identify different haplotypes and clades within this species .
| Collection Site | Haplotype |
|---|---|
| Ubin Island, Singapore | T1 |
| Lim Chu Kang, Singapore | T1 |
| Pasir Ris Park, Singapore | T1 |
| Batu Laut, Selangor, Malaysia | T1 |
Product Specs
Form
Lyophilized powder
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized preparation.
Note: While we will prioritize shipping the format currently in stock, please specify your format preference in order remarks for customized preparation.
Lead Time
Delivery times vary depending on the purchase method and location. Please consult your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Note: All proteins are shipped with standard blue ice packs unless dry ice shipping is requested in advance. Additional fees apply for dry ice shipping.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50%, which can serve as a guideline.
Shelf Life
Shelf life depends on several factors: storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during the manufacturing process.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
fabH; Grc0000083-oxoacyl-[acyl-carrier-protein] synthase 3; EC 2.3.1.180; 3-oxoacyl-[acyl-carrier-protein] synthase III; Beta-ketoacyl-ACP synthase III; KAS III
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-330
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Gracilaria tenuistipitata var. liui (Red alga)
Target Names
fabH
Target Protein Sequence
MQGAQIISIG SAVPDLCINN KEISQIVETS DEWIVTRTGI QERRVLTGTN KSVVDLAYSA
AMKALDKIHM DPLEIDLIIL ATSSPNDLFG SASQVQAKIG AARAVAFDLT AACSGFVLAI
VTATQFIQNG SYKNILIIGA DVLSKWIDWS DRKTCILFGD GAGAAIIQAC YEEDNAILGF
QLNTDGKQYK KLSITYKENN YSLNTLKLFQ GQFQYISMNG KEVYKFAVSK VPASIIKCLN
ALHLSIQDVN WLLLHQANKR ILQAVANRLS VDNYKIISNL SKYGNTSAAS IPLALDEAWQ
NNQIAKEDII VISGFGAGLT WGTVVIKWKC
Uniprot No.
Target Background
Function
This enzyme catalyzes the condensation reaction in fatty acid synthesis by adding two carbons from malonyl-ACP to an acyl acceptor. It initiates fatty acid synthesis by catalyzing the first condensation reaction, thus playing a crucial role in regulating overall fatty acid production. It exhibits both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity dictates the biosynthesis of branched-chain and/or straight-chain fatty acids.
Protein Families
FabH family
Subcellular Location
Plastid, chloroplast.
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