Recombinant Hadronyche versuta Delta-hexatoxin-Hv1b

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Cat. No.

BT31330

Source

Yeast

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31330

Source

E.coli

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31330

Source

E.coli

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31330

Source

Baculovirus

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31330

Source

Mammalian cell

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Purity

>85% (SDS-PAGE)

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Description

Definition and Biological Origin

Delta-hexatoxin-Hv1b is a 42-residue peptide toxin encoded by an intronless gene in H. versuta . It shares 67% sequence identity with its paralog, Delta-hexatoxin-Hv1a (δ-ACTX-Hv1a), but exhibits distinct pharmacological properties, including reduced vertebrate toxicity and no insecticidal activity . The recombinant form (rHv1b) is produced via heterologous expression in yeast systems, enabling scalable production for research and therapeutic applications .

Recombinant Production

rHv1b is synthesized using a yeast (Saccharomyces cerevisiae) expression system, with protocols optimized for high yield and purity (>85% by SDS-PAGE) .

Key Production Parameters:

Expression Vector: pQE30 plasmid with E. coli SHuffle system .
Purification: Reverse-phase HPLC with C₁₈ columns .
Storage: Lyophilized at -80°C; reconstituted in deionized water with 50% glycerol for stability .

Functional Properties

rHv1b modulates tetrodotoxin-sensitive (TTX-S) Naᵥ channels in vertebrates but lacks insecticidal activity .

Electrophysiological Effects:

Parameter	rHv1b Activity
Vertebrate Naᵥ Channels	Slows inactivation kinetics (EC₅₀ ≈ 150 nM in rat dorsal root ganglion neurons)
Insect Naᵥ Channels	No significant effect at concentrations up to 1 µM
Selectivity	Binds neurotoxin receptor site 3 (Naᵥ domain IV S3/S4 loop)

Comparative Analysis with δ-Hexatoxin-Hv1a

Feature	Hv1b	Hv1a
Sequence Identity	67%	Reference
Insecticidal Activity	Absent	Potent (LD₅₀ < 10 nM in insects)
Vertebrate Potency	15–30-fold lower than Hv1a	High (EC₅₀ ≈ 5 nM)
Therapeutic Potential	Research tool for Naᵥ channel studies	Antivenom target

Applications and Research Significance

Mechanistic Studies: Used to probe Naᵥ channel inactivation dynamics and subtype-specific interactions .
Safety Profile: Reduced vertebrate toxicity makes it safer for in vitro assays compared to Hv1a .
Biotechnological Utility: Serves as a template for engineering ion channel modulators with tailored selectivity .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery time varies by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Contact us in advance for dry ice shipping, which incurs extra fees.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect the contents. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

Delta-hexatoxin-Hv1b; Delta-HXTX-Hv1b; Delta-atracotoxin-Hv1b; Delta-ACTX-Hv1b

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-42

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Hadronyche versuta (Blue mountains funnel-web spider) (Atrax versutus)

Target Protein Sequence

CSRSDGWCGK TEDCCCPMKC IKAWYKQNGN CQNTISAIWK NC

Uniprot No.

P81885

Target Background

Function

Lethal neurotoxin. Slows inactivation of tetrodotoxin-sensitive voltage-gated sodium channels (Nav) by binding to site 3, causing repetitive firing in autonomic and motor nerve fibers.

Protein Families

Delta-atracotoxin family

Subcellular Location

Secreted.

Tissue Specificity

Expressed by the venom gland.

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Recombinant Hadronyche versuta Delta-hexatoxin-Hv1b

Description

Definition and Biological Origin

Recombinant Production

Key Production Parameters:

Functional Properties

Electrophysiological Effects:

Comparative Analysis with δ-Hexatoxin-Hv1a

Applications and Research Significance

Product Specs

Target Background

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