Recombinant Haemophilus ducreyi Na (+)-translocating NADH-quinone reductase subunit E (nqrE)
Description
Recombinant Production and Characterization
Recombinant NqrE subunits are typically expressed in Escherichia coli with affinity tags (e.g., His-tag) for purification. For example:
Notes:
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Purification methods often involve Ni-NTA affinity chromatography .
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Structural studies of homologous NqrE subunits in V. cholerae reveal conserved domains critical for binding cofactors like FMN and iron-sulfur clusters .
Role in Bacterial Pathogenesis
While H. ducreyi NqrE has not been directly studied, Na(+)-NQR systems in pathogens contribute to virulence by:
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Stress Adaptation: Supporting survival under low-oxygen or anaerobic conditions encountered during infection .
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Bioenergetics: Maintaining ion gradients essential for nutrient import and efflux of antimicrobial agents .
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Link to Virulence Factors: In H. ducreyi, stationary-phase gene regulation (mediated by proteins like Hfq) controls virulence determinants such as cytotoxins and adhesion proteins . Though NqrE is not explicitly linked here, its role in energy metabolism may indirectly influence virulence gene expression.
Research Gaps and Comparative Insights
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Homology: H. ducreyi NqrE shares sequence homology with Vibrio NqrE (e.g., conserved FMN-binding domains), suggesting similar mechanistic roles .
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Experimental Data: No direct studies on H. ducreyi NqrE exist in the literature reviewed. Current knowledge relies on:
Technical Applications
Recombinant NqrE has potential uses in:
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have a specific format preference, please indicate your requirement when placing the order. We will accommodate your request if possible.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance as additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: hdu:HD_0383
STRING: 233412.HD0383
