Recombinant Haemophilus influenzae Putative TRAP transporter small permease protein HI_0051 (HI_0051)
Description
Functional Insights
HI_0051 operates via an "elevator-with-an-operator" mechanism, driven by sodium gradients :
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Substrate specificity: Transports N-acetylneuraminate (sialic acid), a virulence factor for H. influenzae .
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SiaP interaction: Binds the substrate-binding protein SiaP with micromolar affinity (KD ≈ 1–10 µM) .
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Key residues: R484, D480, and K236 form a salt bridge network essential for SiaP binding and transporter activity . Mutations (e.g., R484E) reduce bacterial growth by 50–80% .
Biochemical Characteristics
Commercial preparations (e.g., Creative BioMart Cat# RFL36242HF) exhibit:
Research Applications
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Mechanistic studies: Used to resolve TRAP transporter dynamics via cryo-EM and mutagenesis .
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Pathogenicity research: Links sialic acid uptake to H. influenzae survival in host environments .
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Protein interaction assays: Facilitates in vitro binding studies with SiaP and homologs .
Evolutionary and Genomic Context
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order. We will fulfill your request whenever possible.
Note: All protein shipments are standardly accompanied by blue ice packs. If you require dry ice shipping, please inform us in advance. Additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: hin:HI0051
STRING: 71421.HI0051
