Recombinant Haemophilus somnus Elongation factor Ts (tsf)

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Cat. No.

BT33672

Source

Yeast

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33672

Source

E.coli

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33672

Source

E.coli

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33672

Source

Baculovirus

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Cat. No.

BT33672

Source

Mammalian cell

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Purity

>85% (SDS-PAGE)

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Description

Introduction

Recombinant Haemophilus somnus (reclassified as Histophilus somni) Elongation Factor Ts (EF-Ts), encoded by the tsf gene, is a critical protein in bacterial translation elongation. EF-Ts functions as a nucleotide exchange factor for Elongation Factor Tu (EF-Tu), facilitating the regeneration of GTP-bound EF-Tu for subsequent rounds of aminoacyl-tRNA delivery to the ribosome. Its recombinant form is produced via heterologous expression systems, enabling detailed biochemical and functional studies.

Genetic Basis

The tsf gene in H. somni is part of the str operon, which includes ribosomal proteins and elongation factors¹.
Recombinant EF-Ts is generated by cloning the tsf gene into expression vectors (e.g., pET or pGEX systems) and expressing it in Escherichia coli².

Functional Role in Translation

EF-Ts ensures efficient protein synthesis by:

Regulating EF-Tu Activity: Catalyzing the release of GDP from EF-Tu and promoting GTP binding⁴.
Stress Response: Under heat shock, EF-Tu forms complexes with molecular chaperones like Hsp33, where EF-Ts modulates EF-Tu stability and aggregation⁴.

Interaction with Chaperones

Hsp33-Mediated Unfolding: EF-Ts enhances EF-Tu’s susceptibility to Hsp33, leading to partial unfolding and protease susceptibility under oxidative stress⁴.
Thermal Stability: Recombinant EF-Ts stabilizes EF-Tu at elevated temperatures (40–42°C), delaying aggregation⁴.

Biochemical Assays

Parameter	Value	Method
Binding Affinity (EF-Tu:EF-Ts)	$K_d = 0.5 \, \mu\text{M}$	Surface Plasmon Resonance⁴
Optimal pH	7.5–8.0	Enzymatic Activity Assay⁴

Applications in Research

Antimicrobial Target: EF-Ts is a potential target for inhibitors disrupting bacterial translation[^7].
Vaccine Development: While not directly tested for H. somni, EF-Tu/EF-Ts complexes have been explored as vaccine candidates in related pathogens⁵[^6].

Challenges and Future Directions

Structural Studies: No crystal structure of H. somni EF-Ts is available; homology modeling is currently used³².
Host-Pathogen Interactions: Further research is needed to elucidate EF-Ts’ role in H. somni virulence and immune evasion⁶¹.

References

This synthesis integrates molecular, functional, and applied perspectives on recombinant H. somni EF-Ts, emphasizing its role in translation and potential biotechnological applications.

Footnotes

[Horizontal Gene Transfer in H. somni (PMC, 2011)]¹ ↩ ↩² ↩³ ↩⁴
[US20040018503A1: Recombinant Haemophilus constructs]² ↩ ↩² ↩³
[US6355450B1: Genomic sequence of Haemophilus]³ ↩ ↩² ↩³
[Molecular Effects of EF-Ts on EF-Tu (Biology, 2021)]⁴ ↩ ↩² ↩³ ↩⁴ ↩⁵ ↩⁶ ↩⁷ ↩⁸ ↩⁹
[Humoral Response to H. somni rHsp60 (Vet Med, 2015)]⁵ ↩ ↩²
[Virulence Factors of H. somnus (VT Dissertation, 2005)]⁶ ↩ ↩²

Product Specs

Form

Lyophilized powder. We will ship the available format, but you can specify your preference when ordering.

Lead Time

Delivery times vary. Consult your local distributor for specifics. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag preference, please inform us.

Synonyms

tsf; HSM_1018Elongation factor Ts; EF-Ts

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-283

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Histophilus somni (strain 2336) (Haemophilus somnus)

Target Names

tsf

Target Protein Sequence

MAEITAALVK ELRERTGAGM MECKKALVEA NGDIELAIDN MRKSGQAKAA KKAGRIAAEG VIRVRIGSGF GVLVELNCET DFVAKDTGFL GLADEVADYA LANKGTTIDT LATHFEDKRA ALVAKIGENM TIRRVQYLEG DVIAQYLHGA KIGVLVAGSG SEEELRKVAM HVAASKPEFV NPEDVSAEVV EHERQIQIDI AINSGKPKEI AEKMVEGRMK KFTGEVSLTG QAFVMDPSVS VGDYLKSVNT SVTNFIRLEV GEGIEKVEED FAAEVAKITG NNA

Uniprot No.

B0UT99

Target Background

Function

Associates with the EF-Tu.GDP complex, promoting GDP-GTP exchange. Remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex until GTP hydrolysis on the ribosome.

Database Links

KEGG: hsm:HSM_1018

Protein Families

EF-Ts family

Subcellular Location

Cytoplasm.

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Recombinant Haemophilus somnus Elongation factor Ts (tsf)

Description

Introduction

Genetic Basis

Functional Role in Translation

Interaction with Chaperones

Biochemical Assays

Applications in Research

Challenges and Future Directions

References

Footnotes

Product Specs

Target Background

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