Recombinant Helianthus annuus NAD (P)H-quinone oxidoreductase subunit 1, chloroplastic (ndhA)
Description
Introduction to Recombinant Helianthus annuus NAD(P)H-Quinone Oxidoreductase Subunit 1, Chloroplastic (ndhA)
Recombinant Helianthus annuus NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic (ndhA) is a genetically engineered protein derived from the common sunflower (Helianthus annuus). This enzyme is a critical subunit of the chloroplast NAD(P)H dehydrogenase-like (NDH) complex, which facilitates photosystem I (PSI) cyclic electron transport and chlororespiration . Produced in E. coli expression systems, the recombinant form retains functional properties of the native protein and is widely used in biochemical research to study chloroplast metabolism and stress responses .
Functional Roles in Chloroplasts
The ndhA subunit is integral to the NDH complex, which performs two primary roles:
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Cyclic Electron Transport (CET): Facilitates electron recycling around PSI, balancing ATP/NADPH production during photosynthesis .
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Chlororespiration: Mediates plastoquinone reduction, linking photosynthetic and respiratory pathways .
Key functional insights:
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RNA Stability & Splicing: ndhA transcript stability is regulated by pentatricopeptide repeat (PPR) proteins (e.g., SOT1) and CRS2-associated factors (CAFs), which inhibit RNA degradation and enhance splicing efficiency .
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Stress Adaptation: The NDH complex, including ndhA, is critical for plant resilience under high light, drought, and temperature extremes .
Experimental Applications
Recombinant ndhA is utilized in:
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ELISA and Protein Interaction Studies: Used to investigate binding partners like CAF1/2 and SOT1 .
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Structural Biology: Crystallography and mutagenesis to map substrate-binding sites (e.g., quinone interaction residues) .
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Biotechnological Engineering: Artificial relocation of ndhA into chloroplasts enhances stress tolerance in engineered plants .
Key Research Findings
Recent studies highlight:
Product Specs
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Target Background
NDH (NAD(P)H-quinone oxidoreductase) facilitates electron transfer from NAD(P)H to plastoquinones within the photosynthetic electron transport chain, potentially also contributing to chloroplast respiration. This process involves FMN and iron-sulfur (Fe-S) centers as intermediate electron carriers. In this species, plastoquinone is believed to be the primary electron acceptor. NDH couples this redox reaction to proton translocation, generating a proton gradient that conserves energy.
KEGG: han:4055622
