Recombinant Helicobacter acinonychis ATP synthase subunit a (atpB)
Description
Genomic Context and Evolutionary Insights
Comparative Genomics:
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H. acinonychis diverged from H. pylori ~200,000 years ago via a host jump to large felines .
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The atpB gene is highly conserved between H. acinonychis and H. pylori, with ~92% sequence identity .
Key Mutations:
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Strain Sheeba retains functional atpB despite extensive gene fragmentation in other loci (e.g., vacA, cagPAI) .
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No frameshifts or premature stop codons observed in atpB, unlike ~8% of other genes in H. acinonychis .
Functional Role in Bacterial Physiology
ATP Synthase Complex:
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Subunit a partners with subunits b, b', and c to form the F0 proton channel .
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Critical for maintaining proton motive force and ATP synthesis in acidic gastric environments .
Target for Pathogenicity Studies:
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Used to investigate ATP synthase inhibition as a potential therapeutic strategy .
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Enables comparative studies of energy metabolism in Helicobacter species .
Biotechnological Use:
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Serves as an antigen in ELISA for detecting H. acinonychis infections in captive felines .
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Facilitates structural biology efforts to map bacterial ATP synthase mechanisms .
Future Directions
Product Specs
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Target Background
Function: A key component of the proton channel, directly involved in proton translocation across the membrane.
KEGG: hac:Hac_1196
STRING: 382638.Hac_1196
