Recombinant Helicobacter pylori Acyl carrier protein (acpP)

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Cat. No.

BT31375

Source

Yeast

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31375

Source

E.coli

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31375

Source

E.coli

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31375

Source

Baculovirus

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

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Cat. No.

BT31375

Source

Mammalian cell

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Purity

>85% (SDS-PAGE)

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Description

Definition and Biological Role

Recombinant Helicobacter pylori acyl carrier protein (acpP) is a genetically engineered form of the native acpP protein, a critical component in bacterial fatty acid biosynthesis. The acpP gene in H. pylori encodes an 84-amino-acid protein with a molecular mass of 9,838 Da . ACP functions as a scaffold for fatty acid intermediates during phospholipid and membrane lipid synthesis, transferring acyl groups via its 4′-phosphopantetheine prosthetic group .

Cloning and Expression

Gene Amplification: The acpP gene is PCR-amplified using primers containing BamHI restriction sites .
Vector Systems: Cloned into plasmid pET-11a and expressed in E. coli BL21(DE3) pLysS, yielding soluble recombinant hpACP .

Table 1: Key Cloning Parameters

Parameter	Detail	Source
Plasmid	pET-11a (Novagen)
Host Strain	E. coli BL21(DE3) pLysS
Inducible Expression	IPTG (1 mM, 16–20 h at 16°C)

Role in Fatty Acid Biosynthesis

Recombinant hpACP is essential for:

Acyl Chain Transfer: Delivers malonyl and acyl intermediates to enzymes like FabD (MCAT) and FabX during type II fatty acid synthesis .
Unsaturated Fatty Acid Synthesis: Collaborates with FabX to introduce cis-3 double bonds in unsaturated fatty acids, critical for membrane fluidity .

Interaction Studies

MCAT-ACP Binding: Surface plasmon resonance (SPR) assays show hpACP binds hpMCAT with a K<sub>D</sub> of 12.3 µM .
FabX-ACP Complex: Crystal structures reveal hpACP docks into FabX’s positively charged groove, facilitating electron transfer via a [4Fe-4S] cluster .

Table 2: Key Interaction Metrics

Interaction Partner	Method	Affinity/Result	Source
hpMCAT	SPR	K<sub>D</sub> = 12.3 µM
FabX	X-ray crystallography	Dynamic acyl-ACP docking

Drug Target Validation

Antibacterial Development: hpACP’s role in lipid biosynthesis makes it a target for inhibitors disrupting acyl transfer .
Structural Insights: Solved hpMCAT-hpACP complexes guide rational drug design .

Diagnostic and Vaccine Development

Antigenicity: Recombinant hpACP elicits immune responses in murine models, suggesting utility in vaccine formulations .
Serological Detection: Recognized by antibodies in H. pylori-infected patient sera .

Challenges and Future Directions

Functional Redundancy: H. pylori encodes multiple ACP homologs (e.g., acp1), complicating knockout studies .
Host-Pathogen Interactions: hpACP-modified lipids may evade Toll-like receptor 2 (TLR2)-mediated immunity, aiding bacterial persistence .

Product Specs

Form

Lyophilized powder. We will ship the format in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchasing method and location. Consult local distributors for specific times. Proteins are shipped with blue ice packs. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type, please inform us for preferential development.

Synonyms

acpP; HPG27_519Acyl carrier protein; ACP

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-78

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Helicobacter pylori (strain G27)

Target Names

acpP

Target Protein Sequence

MALFEDIQAV IAEQLNVDAA QVTPEAEFVK DLGADSLDVV ELIMALEEKF GVEIPDEQAE KIVNVGDVVK YIEDNKLA

Uniprot No.

B5Z6T1

Target Background

Function

Carries the growing fatty acid chain during fatty acid biosynthesis.

Database Links

KEGG: hpg:HPG27_519

Protein Families

Acyl carrier protein (ACP) family

Subcellular Location

Cytoplasm.

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Recombinant Helicobacter pylori Acyl carrier protein (acpP)

Description

Definition and Biological Role

Cloning and Expression

Table 1: Key Cloning Parameters

Role in Fatty Acid Biosynthesis

Interaction Studies

Table 2: Key Interaction Metrics

Drug Target Validation

Diagnostic and Vaccine Development

Challenges and Future Directions

Product Specs

Target Background

Quick Inquiry

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