Recombinant Heliobacterium modesticaldum ATP synthase subunit delta (atpH)

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Cat. No.

BT47104

Source

Yeast

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Purity

>85% (SDS-PAGE)

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Cat. No.

BT47104

Source

E.coli

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Purity

>85% (SDS-PAGE)

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Cat. No.

BT47104

Source

E.coli

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Purity

>85% (SDS-PAGE)

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Cat. No.

BT47104

Source

Baculovirus

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Purity

>85% (SDS-PAGE)

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Cat. No.

BT47104

Source

Mammalian cell

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Purity

>85% (SDS-PAGE)

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Description

Introduction to ATP Synthase in Heliobacterium modesticaldum

The ATP synthase of Heliobacterium modesticaldum, a thermophilic anaerobic phototrophic bacterium, is an F-type rotary nanomachine critical for energy conversion. This enzyme comprises a hydrophilic F $_1$ subcomplex (α $_3$ β $_3$ γδε) and a hydrophobic F $_O$ subcomplex (a, b $_2$ , c $_{10–15}$ ) . The δ subunit (encoded by atpH) is part of the peripheral stalk connecting F $_1$ and F $_O$ , playing a structural and regulatory role in coupling proton translocation to ATP synthesis .

Biochemical and Genetic Context of atpH in H. modesticaldum

Gene cluster: The ATP synthase operon in H. modesticaldum includes atpH alongside other subunits (α, β, γ, ε, a, b, c) .
Functional role: The δ subunit ensures efficient energy coupling during phototrophic growth, which is critical given the bacterium’s reliance on cyclic electron transport for ATP synthesis .

Table 1: Key Features of H. modesticaldum ATP Synthase Subunits

Subunit	Gene	Role	Expression System Used
δ	atpH	Peripheral stalk stabilization	Native purification
α/β	atpA/B	Catalytic nucleotide binding	Sucrose gradient
c-ring	atpE	Proton translocation	β-DDM solubilization

Purification and Characterization of Native δ Subunit

The δ subunit co-purifies with the holoenzyme using:

Detergent solubilization: β-DDM and OG maintain enzyme integrity .
Sucrose density gradient centrifugation: Isolates intact F $_1$ F $_O$ complexes .
Western blotting: Polyclonal antibodies confirm subunit identity .

Despite successful native purification, recombinant production of δ remains unreported. Heterologous systems (e.g., E. coli) used for related Firmicutes could be adapted, leveraging vectors like pAL95R with cbp2 or eno promoters .

Research Gaps and Future Directions

Recombinant expression: No studies yet describe atpH cloning, affinity tagging (e.g., His-tag), or activity assays for the isolated δ subunit.
Functional studies: Role in ATPase regulation under phototrophic vs. fermentative growth conditions warrants investigation .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional charges apply.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, and the lyophilized form is stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

atpH; Helmi_08480; HM1_1101ATP synthase subunit delta; ATP synthase F(1) sector subunit delta; F-type ATPase subunit delta; F-ATPase subunit delta

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-185

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Heliobacterium modesticaldum (strain ATCC 51547 / Ice1)

Target Names

atpH

Target Protein Sequence

MLTGAVARRY AQALLEIGIQ TKTLDALEGE LGRFVEMIGH PELQRFLFHP SIVVAEKKDL VGRLLATGAF SETARAFILL VIDRRRESYF ADIFREFVRL ANKVRNIEEA RVTSAVELAP EQVERLRSQL AAATGKAIVL RMAVDPDLIG GLVVAFGDRI IDGSVAGKIR DLRESLLRSP LPSLS

Uniprot No.

B0TI53

Target Background

Function

F(1)F(0) ATP synthase synthesizes ATP from ADP using a proton or sodium gradient. F-type ATPases have two structural domains: F(1) (catalytic core) and F(0) (membrane proton channel), connected by a central and a peripheral stalk. ATP synthesis in F(1) is coupled to proton translocation through a rotary mechanism of the central stalk subunits. This protein belongs to the stalk connecting CF(0) to CF(1). It either transmits conformational changes from CF(0) to CF(1) or participates in proton conduction.

Database Links

KEGG: hmo:HM1_1101

STRING: 498761.HM1_1101

Protein Families

ATPase delta chain family

Subcellular Location

Cell membrane; Peripheral membrane protein.

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Recombinant Heliobacterium modesticaldum ATP synthase subunit delta (atpH)

Description

Introduction to ATP Synthase in Heliobacterium modesticaldum

Biochemical and Genetic Context of atpH in H. modesticaldum

Table 1: Key Features of H. modesticaldum ATP Synthase Subunits

Purification and Characterization of Native δ Subunit

Research Gaps and Future Directions

Product Specs

Target Background

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