Recombinant Hepatitis E virus genotype 3 Protein ORF3 (ORF3)

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Cat. No.

BT2430962

Source

Yeast

Synonyms

ORF3; Protein ORF3; pORF3

Purity

>85% (SDS-PAGE)

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Cat. No.

BT2430962

Source

E.coli

Synonyms

ORF3; Protein ORF3; pORF3

Purity

>85% (SDS-PAGE)

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Cat. No.

BT2430962

Source

E.coli

Synonyms

ORF3; Protein ORF3; pORF3

Purity

>85% (SDS-PAGE)

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Cat. No.

BT2430962

Source

Baculovirus

Synonyms

ORF3; Protein ORF3; pORF3

Purity

>85% (SDS-PAGE)

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Cat. No.

BT2430962

Source

Mammalian cell

Synonyms

ORF3; Protein ORF3; pORF3

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant Hepatitis E Virus Genotype 3 Protein ORF3

Recombinant Hepatitis E virus (HEV) genotype 3 Protein ORF3 is a multifunctional phosphoprotein critical for viral replication, immune evasion, and virion release. Expressed in E. coli or baculovirus systems, this 113–115 amino acid protein (varies by genotype) is a zoonotic agent involved in quasi-enveloped virion formation and host-pathogen interactions .

Viroporin Activity and Virion Release

Ion Channel Function: Forms multimeric complexes to mediate ion flux, enabling viral egress .
ESCRT Pathway Interaction: Binds host TSG101 via proline-rich motifs to hijack vesicle formation pathways .
Rescue Experiments: Replacing HEV ORF3 with influenza M2 protein restores virion release, confirming functional conservation .

Immune Evasion Mechanisms

Target Pathway	Mechanism	Impact
TLR3/TLR7	Downregulates signaling via suppression of IRF3 phosphorylation	Impaired type I IFN production
STAT1/STAT3	Blocks nuclear translocation of STAT proteins	Reduced cytokine responses
Microtubule Interactions	Disrupts host cytoskeleton dynamics	Facilitates viral trafficking

Regulation of Host Signaling

MAPK/ERK Activation: Promotes cell survival by modulating energy metabolism and growth factor signaling .
HNF4 Modulation: Phosphorylates hepatocyte nuclear factor 4 (HNF4), altering liver-specific gene expression .

Comparative Antigenicity (ORF3 vs. ORF2)

Feature	ORF3	ORF2
Immunogenic Epitopes	Limited (5% swine sera reactivity)	High (85% reactivity in 461–544 aa)
Diagnostic Utility	Low (used in some human ELISAs)	High (primary target for diagnostics)
Vaccine Potential	Partial protection in primates	Primary component of vaccines

Key Findings:

Genotype 3 ORF3 exhibits weak immunogenicity in swine models, limiting its utility as a standalone vaccine antigen .
Bacterially expressed ORF3 (genotype 3) retains functional domains critical for virion release but lacks strong host antibody responses .

Genotype-Specific Insights

Genotype	ORF3 Feature	Functional Impact
3	Altered PSAP motif alignment	Enhanced lipid membrane association
3	Genotype-specific ERK/STAT signaling	Modulates host tropism and virulence
1/2/4	Stronger TLR suppression	Higher IFN-α/β induction

Genotype 3 ORF3 exhibits distinct amino acid variations (e.g., residues 44–50) linked to zoonotic transmission and quasi-enveloped virion stability .

Vaccine Development

Transcomplementation: Co-expressing ORF3 with ORF2 improves quasi-enveloped virion assembly in vitro .
Antigenic Limitations: ORF3’s low immunogenicity necessitates combination with ORF2 or adjuvants for efficacy .

Antiviral Targets

Viroporin Inhibitors: Small molecules targeting ORF3’s ion channel activity could disrupt viral egress .
ESCRT Pathway Inhibitors: Blocking TSG101-ORF3 interactions may hinder virion release .

Product Specs

Form

Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.

Lead Time

Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to settle the contents. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, we recommend adding 5-50% glycerol (final concentration) and aliquoting at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a guideline.

Shelf Life

Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Upon receipt, store at -20°C/-80°C. Aliquot for multiple uses to prevent repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.
The tag type is determined during the production process. Please specify your required tag type for preferential development.

Synonyms

ORF3; Protein ORF3; pORF3

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-113

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Hepatitis E virus genotype 3 (isolate Swine/United States/swUS1) (HEV-3) (Hepatitis E virus genotype 3 (isolate Swine/United States/Meng))

Target Names

ORF3

Target Protein Sequence

MGSPCALGLF CCCSSCFCLC CPRHRPASRL AAVVGGATAV PAVVSGVTGL ILSPSPSPIF IQPTPSLPMS FHNPGLELAL DSRPAPLAPL GVTSPSAPPL PPVVDLPQLG LRR

Uniprot No.

Q6J8G1

Target Background

Function

This protein may function as a viral regulatory protein modulating mitogenic signaling pathways. It may also play a role in virion morphogenesis and viral pathogenesis, potentially accelerating the processing and secretion of AMBP from hepatocytes.

Protein Families

Hepevirus ORF3 protein family

Subcellular Location

Host cytoplasm.

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Recombinant Hepatitis E virus genotype 3 Protein ORF3 (ORF3)

Description

Introduction to Recombinant Hepatitis E Virus Genotype 3 Protein ORF3

Viroporin Activity and Virion Release

Immune Evasion Mechanisms

Regulation of Host Signaling

Comparative Antigenicity (ORF3 vs. ORF2)

Key Findings:

Genotype-Specific Insights

Vaccine Development

Antiviral Targets

Product Specs

Target Background

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