Recombinant Human Adipogenin (ADIG)
Description
Functional Roles in Adipocytes
ADIG is indispensable for LD formation and adipose tissue function:
Lipid Droplet Biogenesis
ADIG promotes LD growth by:
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Stabilizing Seipin Complexes: ADIG selectively binds dodecameric seipin oligomers, enabling efficient triglyceride (TAG) sequestration .
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Regulating LD Morphology: Overexpression in adipocytes enlarges LDs, while knockout leads to aberrant LD formation (e.g., smaller, fragmented LDs in brown adipose tissue) .
Thermogenesis and Metabolic Regulation
ADIG enhances cold-induced thermogenesis by:
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Boosting Triglyceride Uptake: Overexpression in BAT increases TAG absorption from circulation, supporting heat production .
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Maintaining Core Body Temperature: ADIG-deficient mice show impaired cold tolerance, linked to reduced BAT function .
Genomic and Transcriptional Regulation
ADIG expression is tightly regulated:
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PPARγ Dependency: ADIG is transcriptionally activated by PPARγ, a master adipogenic regulator, during adipogenesis .
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Genomic Associations: Variants near ADIG correlate with BMI-adjusted leptin levels, suggesting a role in obesity-related metabolic traits .
Gain-of-Function Studies
Inducible adipocyte-specific ADIG overexpression in mice yields:
| Parameter | Outcome | Source |
|---|---|---|
| Fat Mass | ↑ BAT, sWAT, and eWAT weights | |
| Lipid Metabolism | ↑ Triglyceride clearance; ↑ cholesterol in BAT | |
| Thermogenesis | Attenuated core body temperature drop (cold exposure) |
Loss-of-Function Studies
ADIG knockout in adipocytes results in:
| Parameter | Outcome | Source |
|---|---|---|
| BAT Morphology | Smaller LDs; impaired BAT expansion | |
| Cold Tolerance | ↓ Core body temperature during cold stress | |
| Triglyceride Uptake | Delayed clearance in BAT |
Mechanistic Insights
ADIG’s role in adipose tissue function is multifaceted:
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Seipin Complex Stabilization: ADIG bridges seipin subunits, preventing aggregation and maintaining dodecameric symmetry .
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Lipid Droplet Assembly: ADIG may act as part of the lipid droplet assembly complex (LDAC), facilitating TAG storage in high-demand tissues .
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Cross-Tissue Metabolic Impact: Overexpression enhances systemic triglyceride metabolism, suggesting therapeutic potential for lipid-related disorders .
Unresolved Questions and Future Directions
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Human Relevance: Most studies use murine models; human ADIG’s role in obesity or metabolic diseases remains unexplored .
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Therapeutic Potential: ADIG modulation could target lipid storage disorders or enhance cold-induced energy expenditure .
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Structural Dynamics: The exact stoichiometry of ADIG-seipin interactions and its evolutionary conservation require further study .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order. We will fulfill your request as much as possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please contact us in advance. Additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
- Studies in mouse indicate the 80 aa SMAF1 protein is involved in adipocyte tissue function or regulation. PMID: 15567149
