Recombinant Human Centriolin (CNTRL), partial
Description
Structure and Function
Centriolin is a relatively novel centriolar protein . Antibodies that are raised against recombinant Centriolin recognize a band of ∼270 kD on Western blots of isolated centrosome fractions . The disruption of the CNTRL gene via CRISPR/Cas9 affects the cell in the same way as the reduction of Centriolin protein by siRNA .
Rootletin interacts with C-Nap1, and similar to C-Nap1, rootletin gets phosphorylated by Nek2 kinase and displaced from centrosomes when mitosis starts . Rootletin forms centriole-associated fibers during the interphase of the cell cycle .
Role in Cell Division
Centriolin functions in both cytokinesis and cell cycle progression . Research indicates that reducing centriolin protein levels leads to cytokinesis defects . Cells treated with Cas9 along with a guide RNA targeting the third exon of CNTRL results in a significant reduction in centriolin protein . A failure of peripheral meiotic spindle migration, large polar body emission, and 2-cell like oocytes can result from the knockdown of centriolin in oocytes with either siRNA or Morpholino micro-injection .
Impact of Centriolin Disruption
The disruption of the CNTRL gene by CRISPR/Cas9 affects the cell in the same way as the reduction of Centriolin protein by siRNA, suggesting that the observed phenotype is not due to an off-target effect of the CRISPR/Cas9 . When cells were depleted of centriolin with siRNA, the cells retained an intact microtubule network, which appeared to function normally in both interphase and mitosis .
Experimental Evidence of Centriolin Function
Product Specs
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Target Background
- CNTRL and FGFR1 are implicated in myeloid and lymphoid malignancies in both human and mouse models. PMID: 23777766
- CEP110 is essential for restoring specific aspects of interphase centrosome architecture post-mitosis and for centrosome function as a microtubule-organizing center (MTOC). PMID: 11956314
- Centriolin is hypothesized to anchor protein complexes crucial for vesicle targeting and fusion, integrating membrane-vesicle fusion with abscission. PMID: 16213214
