Recombinant Human Cytoplasmic polyadenylation element-binding protein 3 (CPEB3)

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Cat. No.

BT1245640

Source

Yeast

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Purity

>85% (SDS-PAGE)

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Cat. No.

BT1245640

Source

E.coli

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Purity

>85% (SDS-PAGE)

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Cat. No.

BT1245640

Source

E.coli

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Purity

>85% (SDS-PAGE)

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Cat. No.

BT1245640

Source

Baculovirus

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Purity

>85% (SDS-PAGE)

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Cat. No.

BT1245640

Source

Mammalian cell

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Purity

>85% (SDS-PAGE)

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Description

Molecular Structure and Domains

CPEB3 contains distinct functional domains essential for its activity:

N-terminal prion-like domain (PrD): Facilitates self-aggregation and phase separation .
RNA recognition motifs (RRM1 and RRM2): Bind cytoplasmic polyadenylation elements (CPEs) in target mRNAs .
C-terminal zinc finger (ZnF) domain: Contains a nuclear export signal (NES) critical for cytoplasmic localization .
Low-complexity motif (LCM; residues 220–242): Mediates protein-protein interactions and granule localization .
SUMOylation sites: Post-translational modifications regulate phase separation and activity .

Table 2: Key Research Findings in Neuronal Contexts

Study Focus	Methodology	Outcome
SUMOylation effects	GFP-tagged mutants	SUMOylation loss disrupts P-body localization
LCM mutagenesis	Luciferase assays	S240-242A mutation abolishes translational repression
Ribozyme inhibition	ASO treatment	Elevated CPEB3 increases synaptic protein translation

Cancer

HCC progression: CPEB3 downregulation correlates with poor prognosis; it suppresses MTDH-driven metastasis .
Mouse models: Cpeb3 knockout increases susceptibility to hepatocarcinogenesis and lung metastasis .

Neurodegeneration

Aberrant phase separation links CPEB3 to ALS-associated proteins (e.g., FUS, TDP-43) .

Reproductive Health

CPEB3 depletion causes oocyte transcriptome disruption, leading to embryonic arrest .

Research Tools and Applications

Recombinant CPEB3 is utilized to study:

Phase separation dynamics: In vitro assays show SUMOylated CPEB3 forms condensates with target mRNAs .
Interactome mapping: Mass spectrometry identifies >1,400 interacting proteins, including EJC and FMRP complexes .
Therapeutic targeting: Ribozyme inhibition elevates CPEB3 levels, enhancing synaptic plasticity without altering baseline mRNA .

Future Directions

Therapeutic strategies: Modulating CPEB3 SUMOylation or LCM interactions could treat neurodegenerative diseases or cancer .
Mechanistic gaps: How SUMOylation precisely regulates phase separation and mRNA release remains unclear .

Product Specs

Form

Lyophilized powder

Note: We will prioritize shipping the format currently in stock. If you require a specific format, please specify this in your order notes.

Lead Time

Delivery times vary depending on the purchase method and location. Please contact your local distributor for precise delivery estimates.

Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Centrifuge the vial briefly before opening to collect the contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our standard glycerol concentration is 50% and may serve as a guideline.

Shelf Life

Shelf life depends on storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized formulations have a 12-month shelf life at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquoting is essential for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing.

The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-698

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Homo sapiens (Human)

Target Names

CPEB3

Target Protein Sequence

MQDDLLMDKS KTQPQPQQQQ RQQQQPQPES SVSEAPSTPL SSETPKPEEN SAVPALSPAA APPAPNGPDK MQMESPLLPG LSFHQPPQQP PPPQEPAAPG ASLSPSFGST WSTGTTNAVE DSFFQGITPV NGTMLFQNFP HHVNPVFGGT FSPQIGLAQT QHHQQPPPPA PAPQPAQPAQ PPQAQPPQQR RSPASPSQAP YAQRSAAAAY GHQPIMTSKP SSSSAVAAAA AAAAASSASS SWNTHQSVNA AWSAPSNPWG GLQAGRDPRR AVGVGVGVGV GVPSPLNPIS PLKKPFSSNV IAPPKFPRAA PLTSKSWMED NAFRTDNGNN LLPFQDRSRP YDTFNLHSLE NSLMDMIRTD HEPLKGKHYP PSGPPMSFAD IMWRNHFAGR MGINFHHPGT DNIMALNNAF LDDSHGDQAL SSGLSSPTRC QNGERVERYS RKVFVGGLPP DIDEDEITAS FRRFGPLVVD WPHKAESKSY FPPKGYAFLL FQEESSVQAL IDACLEEDGK LYLCVSSPTI KDKPVQIRPW NLSDSDFVMD GSQPLDPRKT IFVGGVPRPL RAVELAMIMD RLYGGVCYAG IDTDPELKYP KGAGRVAFSN QQSYIAAISA RFVQLQHNDI DKRVEVKPYV LDDQMCDECQ GTRCGGKFAP FFCANVTCLQ YYCEYCWASI HSRAGREFHK PLVKEGGDRP RHVPFRWS

Uniprot No.

Q8NE35

Target Background

Function

CPEB3 is a sequence-specific RNA-binding protein that functions as a translational repressor in the basal state. Upon neuronal stimulation, it acts as a translational activator. Unlike CPEB1, it does not bind to the cytoplasmic polyadenylation element (CPE) but interacts with a U-rich loop within a stem-loop structure in the target mRNA. CPEB3 is crucial for long-term memory consolidation and maintenance. In its basal state, it represses the translation of glutamate receptors (GRIA2/GLUR2), DLG4, GRIN1, GRIN2A, and GRIN2B. Activation leads to the translational activation of GRIA1 and GRIA2. It also regulates SUMO2 translation, repressing it basally and activating it upon stimulation. CPEB3 binds to the 3'-UTR of TRPV1 mRNA, repressing its translation and maintaining normal thermoception. It binds to actin mRNA, repressing its translation basally and activating it upon stimulation. Furthermore, CPEB3 negatively regulates target mRNA levels by binding TOB1, which recruits CNOT7/CAF1, leading to mRNA deadenylation and decay. Beyond its translational role, CPEB3 inhibits STAT5B transcriptional activation without affecting dimerization or DNA binding, thereby repressing EGFR transcription, impacting learning and memory. Unlike CPEB1, CPEB2, and CPEB4, CPEB3 is not required for cell cycle progression.

Gene References Into Functions

miR-452-3p overexpression promotes cell proliferation and mobility while suppressing apoptosis. It enhances EGFR and pAKT expression but inhibits p21 expression, promoting hepatocellular carcinoma (HCC) cell proliferation and mobility by targeting the CPEB3/EGFR axis. PMID: 29332449
CPEB3 expression correlates positively with tumor progression and malignancy but negatively with protein phosphorylation in its alternatively spliced region. PMID: 27256982
EGFR is involved in miR-107 pathogenesis of HCC via CPEB3. PMID: 26497556
The RRM domain of CPEB3 (as a soluble peptide) exhibits a unique protein conformation compared to canonical RRM domains. PMID: 25066254
NMDA-activated calpain 2 cleaves CPEB3, influencing the activity-related translation of CPEB3-targeted RNAs. PMID: 22711986
The cleaved CPEB3 ribozyme exhibits a secondary structure resembling the HDV ribozyme. PMID: 20524672
A conserved mammalian ribozyme sequence within a CPEB3 gene intron has been identified. PMID: 16990549

Database Links

HGNC: 21746

OMIM: 610606

KEGG: hsa:22849

STRING: 9606.ENSP00000265997

UniGene: Hs.131683

Protein Families

RRM CPEB family

Subcellular Location

Cytoplasm. Nucleus. Cell junction, synapse. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.

Q&A

Basic Research Questions

What experimental approaches confirm CPEB3’s role in mRNA translation regulation?

Methodology:
- RNA immunoprecipitation (RIP) identifies CPEB3-bound mRNAs (e.g., MTDH, GluR2) .
- Luciferase reporter assays validate 3′-UTR binding and translational suppression (e.g., MTDH 3′-UTR linked to luciferase shows reduced activity upon CPEB3 overexpression) .
- Western blotting quantifies target protein suppression (e.g., MTDH, FosB) .

How does CPEB3 influence cancer progression?

Key findings:
- Hepatocellular carcinoma (HCC): CPEB3 suppresses metastasis by inhibiting MTDH-mediated epithelial–mesenchymal transition (EMT) .
- Knockout models: Cpeb3⁻/⁻ mice exhibit increased carcinogen-induced HCC and lung metastasis .
Experimental validation: Use CRISPR/Cas9 knockout or siRNA knockdown in HCC cell lines to assess migration/invasion assays (e.g., Transwell) .

Advanced Research Questions

How do phase separation properties of CPEB3 impact its function in neuronal RNA granules?

Mechanistic insights:
- CPEB3 undergoes liquid-liquid phase separation via hydrophobic interactions, forming dynamic condensates critical for mRNA storage/translation .
- SUMOylation regulates its localization to P bodies, influencing translational repression .
Methodological recommendations:
- Fluorescence recovery after photobleaching (FRAP) to assess condensate dynamics.
- Co-immunoprecipitation (Co-IP) with SUMO-specific antibodies to study post-translational modifications .

What contradictions exist regarding CPEB3’s role in synaptic plasticity vs. addiction?

Key contradictions:
- Synaptic plasticity: CPEB3 maintains long-term memory by activating translation in neuronal granules .
- Addiction: Dominant-negative CPEB1/3 mice show reduced cocaine-induced synaptic depression in the nucleus accumbens .
Resolution strategies:
- Use tissue-specific conditional knockouts (e.g., striatum vs. hippocampus).
- Compare phosphorylation states (CPEB3 activity is modulated by kinase signaling in addiction models) .

How to differentiate CPEB3-specific effects from CPEB1/3 functional overlap?

Experimental design:
- Isoform-specific rescue: Express CPEB3 in CPEB1/3 double-knockout models .
- Target-specific RNAi: Knock down CPEB1 vs. CPEB3 in neuronal cultures and profile translation (e.g., ribosome profiling) .

Table 1: Common Technical Issues in CPEB3 Studies

Challenge	Solution	Key Citations
Non-specific antibody cross-reactivity	Validate antibodies using KO cell lysates in Western blotting
Dynamic localization in P bodies	Use leptomycin B to block nuclear export and track cytoplasmic accumulation
Low endogenous CPEB3 expression	Overexpress tagged CPEB3 in HEK293T (lacks endogenous protein) for Co-IP/MS

Critical Data Contradictions Analysis

MTDH regulation: While CPEB3 suppresses MTDH in HCC , its role in other cancers (e.g., breast) remains untested.
- Hypothesis: Tissue-specific co-factors (e.g., Tob/BTG proteins) may modulate CPEB3 activity .
Neuronal vs. non-neuronal roles: CPEB3 enhances memory but suppresses addiction-related plasticity.
- Experimental approach: Compare RNA targets in hippocampal vs. striatal neurons using CLIP-seq .

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Recombinant Human Cytoplasmic polyadenylation element-binding protein 3 (CPEB3)

Description

Molecular Structure and Domains

Table 2: Key Research Findings in Neuronal Contexts

Cancer

Neurodegeneration

Reproductive Health

Research Tools and Applications

Future Directions

Product Specs

Target Background

Q&A

Basic Research Questions

What experimental approaches confirm CPEB3’s role in mRNA translation regulation?

How does CPEB3 influence cancer progression?

Advanced Research Questions

How do phase separation properties of CPEB3 impact its function in neuronal RNA granules?

What contradictions exist regarding CPEB3’s role in synaptic plasticity vs. addiction?

How to differentiate CPEB3-specific effects from CPEB1/3 functional overlap?

Table 1: Common Technical Issues in CPEB3 Studies

Critical Data Contradictions Analysis

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