Recombinant Human E3 ubiquitin-protein ligase SH3RF1 (SH3RF1), partial
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized preparation.
Lead Time
Delivery times vary depending on the purchasing method and location. Contact your local distributor for precise delivery estimates.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Centrifuge the vial briefly before opening to consolidate contents. Reconstitute the protein in sterile, deionized water to a concentration of 0.1-1.0 mg/mL. For long-term storage, add 5-50% glycerol (final concentration) and aliquot at -20°C/-80°C. Our standard glycerol concentration is 50% and serves as a guideline.
Shelf Life
Shelf life depends on various factors including storage conditions, buffer composition, temperature, and protein stability. Generally, liquid formulations have a 6-month shelf life at -20°C/-80°C, while lyophilized forms have a 12-month shelf life at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during the manufacturing process.
The tag type is finalized during production. If you require a specific tag, please inform us, and we will prioritize its development.
The tag type is finalized during production. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
SH3RF1; KIAA1494; POSH; POSH1; RNF142; SH3MD2; E3 ubiquitin-protein ligase SH3RF1; EC 2.3.2.27; Plenty of SH3s; Protein POSH; RING finger protein 142; RING-type E3 ubiquitin transferase SH3RF1; SH3 domain-containing RING finger protein 1; SH3 multiple domains protein 2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Homo sapiens (Human)
Target Names
SH3RF1
Uniprot No.
Target Background
Function
Recombinant Human E3 ubiquitin-protein ligase SH3RF1 (SH3RF1) exhibits E3 ubiquitin-protein ligase activity. It can catalyze self-ubiquitination in the absence of external substrates. It stimulates the ubiquitination of potassium channel KCNJ1, promoting its dynamin-dependent and clathrin-independent endocytosis. It functions as a scaffold protein, coordinating with MAPK8IP1/JIP1 to organize components of the JNK pathway (including RAC1/2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1, and/or MAPK9/JNK2) into a functional complex for efficient JNK activation. It regulates CD4+ and CD8+ T-cell differentiation, promoting Th1 cell differentiation and regulating MAPK8/JNK1 and MAPK9/JNK2 activation in CD4+ T-cells, and MAPK8/JNK1 activation in CD8+ T-cells. Critically, it plays a role in neocortical neuron migration during brain development, controlling proper migration and proximal cytoplasmic dilation formation in migratory neocortical neurons by regulating RAC1 localization and F-actin assembly. In microbial infections, it plays an essential role in targeting HIV-1 Gag to the plasma membrane, a function dependent on its RING domain and E3 ligase activity.
Gene References Into Functions
- POSH's critical role in epileptic seizure progression through N-methyl-D-aspartate receptor trafficking. PMID: 29057721
- Interaction between SH3RF1's N-terminal SH3 domains and FAT1 protein. PMID: 28129444
- Genome-wide significant association for rs2282987 in CDK6 and consistent association for rs2706748 in SH3RF1. PMID: 23644744
- POSH and Siah2 as mediators of death receptor-mediated apoptosis, suggesting their interaction as a potential cancer therapeutic target. PMID: 21586138
- POSH's role in cell survival in Drosophila and human rheumatoid arthritis synovial fibroblasts. PMID: 20974134
- POSH dissociation from the JNK complex following Akt2 phosphorylation of MLK3, leading to JNK pathway downregulation. PMID: 14504284
- A novel step in HIV biogenesis highlighting hPOSH-mediated ubiquitination's role in protein sorting at the trans-Golgi network. PMID: 15659549
- Cooperation between POSH and ALIX in facilitating efficient virus release. PMID: 19393081
- POSH's inhibition of ROMK channels via enhanced dynamin-dependent and clathrin-independent endocytosis and ROMK channel ubiquitination. PMID: 19710010
- Studies in mice. PMID: 9482736
Database Links
Protein Families
SH3RF family
Subcellular Location
Cytoplasm, perinuclear region. Cell projection, lamellipodium. Golgi apparatus, trans-Golgi network.
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